BMRBj - BMREntryMaster

Found 1 Document (0.499 seconds)

BMRB: 27110

1H, 13C and 15N chemical shifts of HIV-1 gp41 cytoplasmic tail residues 707-751

Authors

Murphy, R., Vlach, J., Samal, A., Saad, J.

Assembly

env45

Entity

1. env45 (polymer, Thiol state: all free), 46 monomers, 5230.686 Da Detail

SRVRQGYSPL SFQTHLPIPR GPDRPEGIEE EGGERDRDRS IRLVNG

Formula weight

5230.686 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 90.5 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.5 % (477 of 527)	89.8 % (255 of 284)	90.5 % (180 of 199)	95.5 % (42 of 44)
Backbone	94.7 % (252 of 266)	95.7 % (89 of 93)	93.9 % (124 of 132)	95.1 % (39 of 41)
Sidechain	87.7 % (264 of 301)	86.9 % (166 of 191)	88.8 % (95 of 107)	100.0 % (3 of 3)
Aromatic	0.0 % (0 of 22)	0.0 % (0 of 11)	0.0 % (0 of 11)
Methyl	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)

1. hiv1 env45

SRVRQGYSPL SFQTHLPIPR GPDRPEGIEE EGGERDRDRS IRLVNG

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 308.000 K, pH 6.000

#	Name	Isotope labeling	Type	Concentration
1	hiv1_env45	[[U-95% 13C; U-95% 15N]]		0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 5 Detail

Release date

2017-05-21

Citation

Solution Structure and Membrane Interaction of the Cytoplasmic Tail of HIV-1 gp41 Protein
Murphy, R.E., Samal, A.B., Vlach, J., Saad, J.S.
Structure (2017), 25, 1708-1718, PubMed 29056482 , DOI 10.1016/j.str.2017.09.010 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 30297 released on 2017-05-24
Title 1H, 13C, 15N chemical shift assignments of the HIV-1 gp41 cytoplasmic tail, residues 752-856

Related entities 1. env45, : 2 : 53 entities Detail

Interaction partners 1. env45, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 3D HNCA