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BMRB: 30297

5VWL

1H, 13C, 15N chemical shift assignments of the HIV-1 gp41 cytoplasmic tail, residues 752-856

Authors

Saad, J.S., Murphy, R.E., Samal, A., Vlach, J.

Assembly

Cytoplasmic Tail of HIV-1 gp41 protein

Entity

1. Cytoplasmic Tail of HIV-1 gp41 protein (polymer, Thiol state: all free), 105 monomers, 12381.38 Da Detail

SLALIWDDLR SLCLFSYHRL RDLLLIVTRI VELLGRRGWE ALKYWWNLLQ YWSQELKNSA VNLLNATAIA VAEGTDRVIE VLQAAYRAIR HIPRRIRQGL ERILL

Formula weight

12381.38 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 87.2 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.2 % (1134 of 1300)	83.4 % (561 of 673)	91.2 % (465 of 510)	92.3 % (108 of 117)
Backbone	92.0 % (578 of 628)	90.6 % (193 of 213)	93.2 % (290 of 311)	91.3 % (95 of 104)
Sidechain	84.5 % (653 of 773)	80.0 % (368 of 460)	90.7 % (272 of 300)	100.0 % (13 of 13)
Aromatic	92.7 % (102 of 110)	100.0 % (55 of 55)	84.0 % (42 of 50)	100.0 % (5 of 5)
Methyl	85.3 % (145 of 170)	78.8 % (67 of 85)	91.8 % (78 of 85)

1. entity 1

SLALIWDDLR SLCLFSYHRL RDLLLIVTRI VELLGRRGWE ALKYWWNLLQ YWSQELKNSA VNLLNATAIA VAEGTDRVIE VLQAAYRAIR HIPRRIRQGL ERILL

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 324 K, pH 6.0, Details 500 uM gp4CTc, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM TCEP, 25 mM DPC, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
1	DPC	natural abundance	25 mM
2	TCEP	natural abundance	1 mM
3	gp4CTc	natural abundance	500 uM
4	sodium chloride	natural abundance	50 mM
5	sodium phosphate	natural abundance	50 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 324 K, pH 6.0, Details 500 uM [U-99% 15N] gp4CTc, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM TCEP, 25 mM DPC, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
6	DPC	natural abundance	25 mM
7	TCEP	natural abundance	1 mM
8	gp4CTc	[U-99% 15N]	500 uM
9	sodium chloride	natural abundance	50 mM
10	sodium phosphate	natural abundance	50 mM

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 324 K, pH 6.0, Details 500 uM [U-99% 13C; U-99% 15N] gp4CTc, 50 mM sodium phosphate, 50 mM sodium chloride, 1 mM TCEP, 25 mM DPC, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
11	DPC	natural abundance	25 mM
12	TCEP	natural abundance	1 mM
13	gp4CTc	[U-99% 13C; U-99% 15N]	500 uM
14	sodium chloride	natural abundance	50 mM
15	sodium phosphate	natural abundance	50 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 5VWL, Strand ID: A Detail

Release date

2017-05-24

Citation

Solution Structure and Membrane Interaction of the Cytoplasmic Tail of HIV-1 gp41 Protein
Murphy, R.E., Samal, A.B., Vlach, J., Saad, J.S.
Structure (2017), 25, 1708-1718, PubMed 29056482 , DOI 10.1016/j.str.2017.09.010 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 27110 released on 2017-05-21
Title 1H, 13C and 15N chemical shifts of HIV-1 gp41 cytoplasmic tail residues 707-751

Related entities 1. Cytoplasmic Tail of HIV-1 gp41 protein, : 1 : 58 entities Detail

Interaction partners 1. Cytoplasmic Tail of HIV-1 gp41 protein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations