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BMRB: 27609

1FNH

Sequence-specific 1H, 13C, and 15N backbone assignment of Fibronectin type III 13

Authors

Zhong, X., Arnolds, O., Krenczyk, O., Gajewski, J., Puetz, S., Herrmann, C., Stoll, R.

Assembly

FN III 13 module

Entity

1. FN III 13 module (polymer, Thiol state: not present), 111 monomers, 12441.74 Da Detail

MGHHHHHHAE NLYFQGHMLE NVSPPRRARV TDATETTITI SWRTKTETIT GFQVDAVPAN GQTPIQRTIK PDVRSYTITG LQPGTDYKIY LYTLNDNARS SPVVIDASTA I

Formula weight

12441.74 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.1 %, Completeness: 36.4 %, Completeness (bb): 63.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	36.4 % (459 of 1260)	19.6 % (127 of 647)	50.0 % (249 of 498)	72.2 % (83 of 115)
Backbone	63.2 % (412 of 652)	42.1 % (93 of 221)	73.1 % (239 of 327)	76.9 % (80 of 104)
Sidechain	16.7 % (119 of 713)	8.2 % (35 of 426)	29.3 % (81 of 276)	27.3 % (3 of 11)
Aromatic	4.0 % (4 of 100)	4.0 % (2 of 50)	4.1 % (2 of 49)	0.0 % (0 of 1)
Methyl	16.7 % (22 of 132)	13.6 % (9 of 66)	19.7 % (13 of 66)

1. FNIII13

MGHHHHHHAE NLYFQGHMLE NVSPPRRARV TDATETTITI SWRTKTETIT GFQVDAVPAN GQTPIQRTIK PDVRSYTITG LQPGTDYKIY LYTLNDNARS SPVVIDASTA I

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	FNIII13	[U-13C; U-15N]	1 mM
2	NaCl	natural abundance	137 mM
3	KCl	natural abundance	2.7 mM
4	Na2HPO4	natural abundance	10 mM
5	KH2PO4	natural abundance	1.4 mM
6	NaN3	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 1.06 ppm, Outliers: 1 Detail

Release date

2018-09-13

Citation

The Structure in Solution of Fibronectin Type III Domain 14 Reveals Its Synergistic Heparin Binding Site
Zhong, X., Arnolds, O., Krenczyk, O., Gajewski, J., Puetz, S., Herrmann, C., Stoll, R.
Biochemistry (2018), 57, 6045-6049, PubMed 30260627 , DOI 10.1021/acs.biochem.8b00771 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 27610 released on 2018-09-13
Title Sequence-specific 1H, 13C, and 15N backbone assignment of Fibronectin type III 14

Related entities 1. FN III 13 module, : 1 : 3 : 208 entities Detail

Interaction partners 1. FN III 13 module, : 91 interactors Detail

More details for 91 interactors identified by 47 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC