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BMRB: 27610

6HNF

Sequence-specific 1H, 13C, and 15N backbone assignment of Fibronectin type III 14

Authors

Zhong, X., Arnolds, O., Krenczyk, O., Gajewski, J., Puetz, S., Herrmann, C., Stoll, R.

Assembly

FN III 14 module

Entity

1. FN III 14 module (polymer, Thiol state: not present), 91 monomers, 10082.51 Da Detail

GHMDAPSNLR FLATTPNSLL VSWQPPRARI TGYIIKYEKP GSPPREVVPR PRPGVTEATI TGLEPGTEYT IYVIALKNNQ KSEPLIGRKK T

Formula weight

10082.51 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 91.5 %, Completeness (bb): 91.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.5 % (982 of 1073)	91.2 % (516 of 566)	91.9 % (387 of 421)	91.9 % (79 of 86)
Backbone	91.6 % (478 of 522)	91.5 % (162 of 177)	91.7 % (244 of 266)	91.1 % (72 of 79)
Sidechain	90.6 % (575 of 635)	89.5 % (348 of 389)	92.1 % (220 of 239)	100.0 % (7 of 7)
Aromatic	65.5 % (38 of 58)	65.5 % (19 of 29)	64.3 % (18 of 28)	100.0 % (1 of 1)
Methyl	94.2 % (98 of 104)	92.3 % (48 of 52)	96.2 % (50 of 52)

1. FNIII14

GHMDAPSNLR FLATTPNSLL VSWQPPRARI TGYIIKYEKP GSPPREVVPR PRPGVTEATI TGLEPGTEYT IYVIALKNNQ KSEPLIGRKK T

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	FNIII14	[U-13C; U-15N]	1 mM
2	NaCl	natural abundance	137 mM
3	KCl	natural abundance	2.7 mM
4	Na2HPO4	natural abundance	10 mM
5	KH2PO4	natural abundance	1.4 mM
6	NaN3	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.93 ppm, Outliers: 3 Detail

Release date

2018-09-13

Citation

The Structure in Solution of Fibronectin Type III Domain 14 Reveals Its Synergistic Heparin Binding Site
Zhong, X., Arnolds, O., Krenczyk, O., Gajewski, J., Puetz, S., Herrmann, C., Stoll, R.
Biochemistry (2018), 57, 6045-6049, PubMed 30260627 , DOI 10.1021/acs.biochem.8b00771 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 27609 released on 2018-09-13
Title Sequence-specific 1H, 13C, and 15N backbone assignment of Fibronectin type III 13

Related entities 1. FN III 14 module, : 1 : 760 entities Detail

Interaction partners 1. FN III 14 module, : 91 interactors Detail

More details for 91 interactors identified by 47 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC