Solution structure of anti-CRISPR protein AcrIF7 from Pseudomonas aeruginosa Phage
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.3 % (706 of 757) | 94.8 % (363 of 383) | 90.0 % (270 of 300) | 98.6 % (73 of 74) |
Backbone | 97.1 % (398 of 410) | 95.8 % (136 of 142) | 97.5 % (196 of 201) | 98.5 % (66 of 67) |
Sidechain | 90.0 % (369 of 410) | 94.2 % (227 of 241) | 83.3 % (135 of 162) | 100.0 % (7 of 7) |
Aromatic | 68.1 % (64 of 94) | 83.0 % (39 of 47) | 52.2 % (24 of 46) | 100.0 % (1 of 1) |
Methyl | 98.5 % (65 of 66) | 100.0 % (33 of 33) | 97.0 % (32 of 33) |
1. AcrIF7
GHMTTFTSIV TTNPDFGGFE FYVEAGQQFD DSAYEEAYGV SVPSAVVEEM NAKAAQLKDG EWLNVSHEASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.6 mM AcrIF7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | AcrIF7 | [U-99% 13C; U-99% 15N] | 0.6 mM | |
2 | HEPES | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 200 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | EDTA | natural abundance | 1 mM |