NMR solution structure of wild type apo hFABP1 at 308 K
HHHHHHVAMS FSGKYQLQSQ ENFEAFMKAI GLPEELIQKG KDIKGVSEIV QNGKHFKFTI TAGSKVIQNE FTVGEECELE TMTGEKVKTV VQLEGDNKLV TTFKNIKSVT ELNGDIITNT MTLGDIVFKR ISKRI
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.0 % (1384 of 1610) | 91.9 % (769 of 837) | 75.5 % (472 of 625) | 96.6 % (143 of 148) |
Backbone | 82.3 % (665 of 808) | 95.0 % (266 of 280) | 68.5 % (270 of 394) | 96.3 % (129 of 134) |
Sidechain | 90.5 % (838 of 926) | 90.3 % (503 of 557) | 90.4 % (321 of 355) | 100.0 % (14 of 14) |
Aromatic | 74.1 % (86 of 116) | 75.9 % (44 of 58) | 72.4 % (42 of 58) | |
Methyl | 96.8 % (151 of 156) | 94.9 % (74 of 78) | 98.7 % (77 of 78) |
1. entity 1
HHHHHHVAMS FSGKYQLQSQ ENFEAFMKAI GLPEELIQKG KDIKGVSEIV QNGKHFKFTI TAGSKVIQNE FTVGEECELE TMTGEKVKTV VQLEGDNKLV TTFKNIKSVT ELNGDIITNT MTLGDIVFKR ISKRISolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 1 mM [U-13C; U-15N] Human liver fatty acid binding protein (hFABP1), 20 mM sodium phosphate, 50 mM sodium chloride, pH 5.5 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium phosphate | natural abundance | 20 mM | |
2 | sodium chloride | natural abundance | 50 mM | |
3 | Human liver fatty acid binding protein (hFABP1) | [U-13C; U-15N] | 1 mM |