Ablb 1b isoform active state
SPNYDKWEME RTDITMKHKL GGGQYGEVYE GVWKKYSLTV AVKTLKEDTM EVEEFLKEAA VMKEIKHPNL VQLLGVCTRE PPFYIITEFM TYGNLLDYLR ECNRQEVNAV VLLYMATQIS SAMEYLEKKN FIHRDLAARN CLVGENHLVK VADFGLSRLM TGDTYTAHAG AKFPIKWTAP ESLAYNKFSI KSDVWAFGVL LWEIATYGMS PYPGIDLSQV YELLEKDYRM ERPEGCPEKV YELMRACWQW NPSDRPSFAE IHQAFETMFQ ESSISDEVEK ELGKQGV
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 24.1 % (834 of 3454) | 23.5 % (420 of 1790) | 17.4 % (235 of 1352) | 57.4 % (179 of 312) |
Backbone | 26.0 % (442 of 1698) | 33.3 % (193 of 579) | 9.1 % (77 of 844) | 62.5 % (172 of 275) |
Sidechain | 21.5 % (435 of 2026) | 18.7 % (227 of 1211) | 25.8 % (201 of 778) | 18.9 % (7 of 37) |
Aromatic | 16.8 % (58 of 346) | 19.1 % (33 of 173) | 10.8 % (18 of 166) | 100.0 % (7 of 7) |
Methyl | 91.3 % (274 of 300) | 91.3 % (137 of 150) | 91.3 % (137 of 150) |
1. entity 1
SPNYDKWEME RTDITMKHKL GGGQYGEVYE GVWKKYSLTV AVKTLKEDTM EVEEFLKEAA VMKEIKHPNL VQLLGVCTRE PPFYIITEFM TYGNLLDYLR ECNRQEVNAV VLLYMATQIS SAMEYLEKKN FIHRDLAARN CLVGENHLVK VADFGLSRLM TGDTYTAHAG AKFPIKWTAP ESLAYNKFSI KSDVWAFGVL LWEIATYGMS PYPGIDLSQV YELLEKDYRM ERPEGCPEKV YELMRACWQW NPSDRPSFAE IHQAFETMFQ ESSISDEVEK ELGKQGVSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.1, Details 250 uM [U-15N] Abl 1b isoform (Active), 25 mM sodium phosphate, 75 mM sodium chloride, 2.5 mM b-mercaptoethanol, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Abl 1b isoform (Active) | [U-15N] | 250 uM | |
2 | sodium phosphate | natural abundance | 25 mM | |
3 | sodium chloride | natural abundance | 75 mM | |
4 | b-mercaptoethanol | natural abundance | 2.5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.1, Details 250 uM [U-100% 13C; U-100% 15N; U-100% 2H] Abl 1b isoform (Active), 25 mM sodium phosphate, 75 mM sodium chloride, 2.5 mM b-mercaptoethanol, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | Abl 1b isoform (Active) | [U-100% 13C; U-100% 15N; U-100% 2H] | 250 uM | |
6 | sodium phosphate | natural abundance | 25 mM | |
7 | sodium chloride | natural abundance | 75 mM | |
8 | b-mercaptoethanol | natural abundance | 2.5 mM |