BMRB: 31029

NMR Structure of Ac-hGal(17-30)NH2, an N-terminally acetylated fragment of the C-terminus of human galanin

Authors

Kraichely, K.N., Hendy, C.M., Parnham, S., Giuliano, M.W.

Assembly

Galanin

Entity

1. Galanin (polymer, Thiol state: not present), 16 monomers, 1543.622 Da Detail

XGNHRSFSDK NGLTSX

Formula weight

1543.622 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.5 %, Completeness: 93.7 %, Completeness (bb): 93.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	93.7 % (74 of 79)	93.7 % (74 of 79)
Backbone	93.3 % (28 of 30)	93.3 % (28 of 30)
Sidechain	93.9 % (46 of 49)	93.9 % (46 of 49)
Aromatic	71.4 % (5 of 7)	71.4 % (5 of 7)
Methyl	100.0 % (3 of 3)	100.0 % (3 of 3)

1. entity 1

XGNHRSFSDK NGLTSX

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 4.6 (±0.02), Details 2 mM Ac-hGal(17-30)-NH2, 4.14 mM [U-2H] sodium acetate, 5.86 mM [U-2H] acetic acid, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ac-hGal(17-30)-NH2	natural abundance		2 mM
2	sodium acetate	[U-2H]		4.14 mM
3	acetic acid	[U-2H]		5.86 mM

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Release date

2022-06-29

Citation

The neuropeptide galanin adopts an irregular secondary structure
Wilkinson, R.E., Kraichely, K.N., Hendy, C.M., Buchanan, L.E., Parnham, S., Giuliano, M.W.
Biochem. Biophys. Res. Commun. (2022), 626, 121-128, PubMed 35994823 , DOI 10.1016/j.bbrc.2022.08.032 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Galanin, : 1 : 7 entities Detail

Interaction partners 1. Galanin, : 5 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail