NMR solution structure of the carbohydrate-binding module family 73 (CBM73) from Cellvibrio japonicus CjLPMO10A
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS4:SG | 1:CYS60:SG |
2 | disulfide | sing | 1:CYS30:SG | 1:CYS36:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 74.3 % (547 of 736) | 73.1 % (272 of 372) | 72.6 % (207 of 285) | 86.1 % (68 of 79) |
Backbone | 87.6 % (352 of 402) | 88.0 % (125 of 142) | 87.2 % (170 of 195) | 87.7 % (57 of 65) |
Sidechain | 62.8 % (247 of 393) | 63.9 % (147 of 230) | 59.7 % (89 of 149) | 78.6 % (11 of 14) |
Aromatic | 7.7 % (8 of 104) | 7.7 % (4 of 52) | 0.0 % (0 of 48) | 100.0 % (4 of 4) |
Methyl | 100.0 % (58 of 58) | 100.0 % (29 of 29) | 100.0 % (29 of 29) |
1. entity 1
MGNCISPVYV DGSSYANNAL VQNNGSEYRC LVGGWCTVGG PYAPGTGWAW ANAWELVRSC QAHHHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.1 mM CBM73, 25 mM sodium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CBM73 | natural abundance | 0.1 (±0.05) mM | |
2 | sodium phosphate | natural abundance | 25 mM | |
3 | sodium chloride | natural abundance | 10 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.1 mM CBM73, 25 mM sodium phosphate, 10 mM sodium chloride, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | CBM73 | natural abundance | 0.1 (±0.05) mM | |
5 | sodium phosphate | natural abundance | 25 mM | |
6 | sodium chloride | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.1 mM [U-15N] CBM73, 25 mM sodium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | CBM73 | [U-15N] | 0.1 (±0.05) mM | |
8 | sodium phosphate | natural abundance | 25 mM | |
9 | sodium chloride | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 0.1 mM [U-13C; U-15N] CBM73, 25 mM sodium phosphate, 10 mM sodium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | CBM73 | [U-13C; U-15N] | 0.1 (±0.05) mM | |
11 | sodium phosphate | natural abundance | 25 mM | |
12 | sodium chloride | natural abundance | 10 mM |