BMRB: 34623

A self-complementary DNA dodecamer duplex contaning 5-hydroxymethylcitosine

Authors

Battistini, F., Dans, P.D., Terrazas, M., Castellazzi, C.L., Portella, G., Labrador, M., Villegas, N., Brun-Heath, I., Gonzalez, C., Orozco, M.

Assembly

DNA (5'-D(*(P*GP*CP*GP*TP*(DH)P*GP*AP*CP*GP*CP*G-3')

Entity

1. DNA (5'-D(*(P*GP*CP*GP*TP*(DH)P*GP*AP*CP*GP*CP*G-3') (polymer, Thiol state: not present), 12 monomers, 3757.378 × 2 Da Detail

CGCGTXGACG CG

Total weight

7514.756 Da

Max. entity weight

3757.378 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.7 %, Completeness: 87.0 %, Completeness (bb): 81.8 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	87.0 % (94 of 108)	87.0 % (94 of 108)
Suger, PO4	81.8 % (63 of 77)	81.8 % (63 of 77)
Nucleobase	100.0 % (31 of 31)	100.0 % (31 of 31)
Aromatic	100.0 % (22 of 22)	100.0 % (22 of 22)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

1. entity 1

CGCGTXGACG CG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 7.0, Details 1 mM DNA (5'-D(*(DC5)P*GP*CP*GP*TP*(DH)P*GP*AP*CP*GP*CP*(DG3))-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*(DC5)P*GP*CP*GP*TP*(DH)P*GP*AP*CP*GP*CP*(DG3))-3')	natural abundance		1 mM

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Release date

2021-06-10

Citation

The Impact of the HydroxyMethylCytosine epigenetic signature on DNA structure and function
Battistini, F., Dans, P.D., Terrazas, M., Castellazzi, C.L., Portella, G., Labrador, M., Villegas, N., Brun-Heath, I., Gonzalez, C., Orozco, M.
PLoS Comput. Biol. (2021), 17, e1009547-e1009547, PubMed 34748533 , DOI 10.1371/journal.pcbi.1009547 , Abstract

Entries sharing articles BMRB: 3 entries Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail