Solution structure of the Family 1 carbohydrate-binding module with mannosylated Ser14
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS8:SG | 1:CYS25:SG |
2 | disulfide | sing | 1:CYS19:SG | 1:CYS35:SG |
3 | ether | sing | 1:SER14:OG | 2:MAN1:C1 |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.3 % (334 of 374) | 97.4 % (185 of 190) | 76.6 % (111 of 145) | 97.4 % (38 of 39) |
Backbone | 82.1 % (174 of 212) | 96.1 % (73 of 76) | 66.7 % (68 of 102) | 97.1 % (33 of 34) |
Sidechain | 99.0 % (190 of 192) | 98.2 % (112 of 114) | 100.0 % (73 of 73) | 100.0 % (5 of 5) |
Aromatic | 100.0 % (36 of 36) | 100.0 % (18 of 18) | 100.0 % (18 of 18) | |
Methyl | 96.7 % (29 of 30) | 93.3 % (14 of 15) | 100.0 % (15 of 15) |
1. Exoglucanase 1
TQSHYGQCGG IGYSGPTVCA SGTTCQVLNP YYSQCLSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5, Details 5 mg/mL CBM, 50 mM [U-2H] sodium acetate, 0.1 mg/mL DSS, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CBM | natural abundance | protein | 5 mg/mL |
2 | DSS | natural abundance | 0.1 mg/mL | |
3 | sodium acetate | [U-2H] | buffer | 50 mM |
4 | H2O | natural abundance | solvent | 90 % |
5 | D2O | [U-2H] | solvent | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5, Details 5 mg/mL CBM, 50 mM [U-2H] sodium acetate, 0.1 mg/mL DSS, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | CBM | natural abundance | protein | 5 mg/mL |
7 | DSS | natural abundance | 0.1 mg/mL | |
8 | sodium acetate | [U-2H] | buffer | 50 mM |
9 | D2O | [U-2H] | solvent | 100 % |