Solution structure of the Family 1 carbohydrate-binding module Q2A mutant with mannosylated Ser3
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS8:SG | 1:CYS25:SG |
2 | disulfide | sing | 1:CYS19:SG | 1:CYS35:SG |
3 | ether | sing | 1:SER3:OG | 2:MAN1:C1 |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.7 % (333 of 367) | 99.5 % (184 of 185) | 77.8 % (112 of 144) | 97.4 % (37 of 38) |
Backbone | 84.0 % (178 of 212) | 98.7 % (75 of 76) | 68.6 % (70 of 102) | 97.1 % (33 of 34) |
Sidechain | 100.0 % (185 of 185) | 100.0 % (109 of 109) | 100.0 % (72 of 72) | 100.0 % (4 of 4) |
Aromatic | 100.0 % (36 of 36) | 100.0 % (18 of 18) | 100.0 % (18 of 18) | |
Methyl | 100.0 % (32 of 32) | 100.0 % (16 of 16) | 100.0 % (16 of 16) |
1. Exoglucanase 1
TASHYGQCGG IGYSGPTVCA SGTTCQVLNP YYSQCLSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5, Details 5 mg/mL CBM-Q2A, 50 mM [U-2H] sodium acetate, 0.1 mg/mL DSS, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CBM-Q2A | natural abundance | protein | 5 mg/mL |
2 | DSS | natural abundance | 0.1 mg/mL | |
3 | sodium acetate | [U-2H] | buffer | 50 mM |
4 | H2O | natural abundance | solvent | 90 % |
5 | D2O | [U-2H] | solvent | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5, Details 5 mg/mL CBM-Q2A, 50 mM [U-2H] sodium acetate, 0.1 mg/mL DSS, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | CBM-Q2A | natural abundance | protein | 5 mg/mL |
7 | DSS | natural abundance | 0.1 mg/mL | |
8 | sodium acetate | [U-2H] | buffer | 50 mM |
9 | D2O | [U-2H] | solvent | 100 % |