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BMRB: 50218

6YI2

UvrD_CTD

Authors

Kawale, A.A., Burmann, B.M.

Assembly

UvrD CTD

Entity

1. UvrD CTD (polymer, Thiol state: not present), 76 monomers, 8438.519 Da Detail

RLRATVSRPV SHQRMGTPMV ENDSGYKLGQ RVRHAKFGEG TIVNMEGSGE HSRLQVAFQG QGIKWLVAAY ARLESV

Formula weight

8438.519 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 88.4 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.4 % (776 of 878)	86.4 % (393 of 455)	93.7 % (312 of 333)	78.9 % (71 of 90)
Backbone	95.4 % (431 of 452)	93.7 % (149 of 159)	96.8 % (212 of 219)	94.6 % (70 of 74)
Sidechain	81.9 % (404 of 493)	81.1 % (240 of 296)	90.1 % (163 of 181)	6.3 % (1 of 16)
Aromatic	83.3 % (50 of 60)	83.3 % (25 of 30)	82.8 % (24 of 29)	100.0 % (1 of 1)
Methyl	97.4 % (76 of 78)	94.9 % (37 of 39)	100.0 % (39 of 39)

1. entity 1

RLRATVSRPV SHQRMGTPMV ENDSGYKLGQ RVRHAKFGEG TIVNMEGSGE HSRLQVAFQG QGIKWLVAAY ARLESV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 6YI2, Strand ID: A Detail

Release date

2020-03-29

Citation

Publisher Correction: UvrD helicase-RNA polymerase interactions are governed by UvrD's carboxy-terminal Tudor domain
Kawale, A.A., Burmann, B.M.
Commun. Biol. (2020), 3, 661-661, PubMed 33154434 , DOI 10.1038/s42003-020-01426-x , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 50219 released on 2020-03-29
Title Mfd_RID

Related entities 1. UvrD CTD, : 1 : 2 : 1 : 14 entities Detail

Interaction partners 1. UvrD CTD, : 12 interactors Detail

More details for 12 interactors identified by 5 citations

Experiments performed 14 experiments Detail

1 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

4 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

5 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

6 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

7 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

8 3D H(CCO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

9 2D HBCBCGCDHD

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

10 2D HBCBCGCDCEHE

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

11 15N-(1H) NOE

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

12 3D HCACO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

13 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

14 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details [KH2PO4 ]: 13.3 mM [K2HPO4 ]: 0.7 mM

#	Name	Isotope labeling	Type	Concentration
1	UvrD_CTD	[U-100% 13C; U-100% 15N]		650 uM
2	potassium phosphate buffer	natural abundance		20 mM
3	KCl	natural abundance		50 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr50218_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr50218_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	868		98.7 (chain: 1, length: 76)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 76, Sequence coverage: 98.7%
- Total number of assignments
  - ¹H chemical shifts: 482
  - ¹³C chemical shifts: 315
  - ¹⁵N chemical shifts: 71
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords UvrD, Tudor, TCR, DNA repair