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BMRB: 50525

transmembrane domain of PD-L1

Authors

Maorong, W., Yunlei, C., Bo, O.

Assembly

PD-L1-TM monomer

Entity

1. PD-L1-TM monomer (polymer, Thiol state: all free), 59 monomers, 6561.547 Da Detail

AHPPNERTHL VILGAILLAL GVALTFIFRL RKGRLLDVKK SGIQDTNSKK QSDTHLEET

Formula weight

6561.547 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 79.9 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.9 % (561 of 702)	83.5 % (303 of 363)	73.4 % (201 of 274)	87.7 % (57 of 65)
Backbone	96.0 % (336 of 350)	95.8 % (115 of 120)	95.4 % (165 of 173)	98.2 % (56 of 57)
Sidechain	68.1 % (277 of 407)	77.4 % (188 of 243)	56.4 % (88 of 156)	12.5 % (1 of 8)
Aromatic	12.5 % (4 of 32)	25.0 % (4 of 16)	0.0 % (0 of 16)
Methyl	90.7 % (78 of 86)	90.7 % (39 of 43)	90.7 % (39 of 43)

1. entity 1

AHPPNERTHL VILGAILLAL GVALTFIFRL RKGRLLDVKK SGIQDTNSKK QSDTHLEET

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.5 mM 15N-13C-2H-PD-L1-TM, 30 mM DMPC, 60 mM DHPC

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.5 mM
2	MES	natural abundance		25 mM
3	DMPC	natural abundance		30 mM
4	DHPC	natural abundance		60 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.5 mM 15N-13C-PD-L1-TM, 30 mM 2H-DMPC, 60 mM 2H-DHPC

#	Name	Isotope labeling	Type	Concentration
5	entity_1	[U-13C; U-15N]		0.5 mM
6	MES	natural abundance		25 mM
7	DMPC	[U-2H]		30 mM
8	DHPC	[U-2H]		60 mM

Release date

2020-10-20

Citation

PD-L1 degradation is regulated by electrostatic membrane association of its cytoplasmic domain
Wen, M., Cao, Y., Wu, B., Xiao, T., Cao, R., Wang, Q., Liu, X., Xue, H., Yu, Y., Lin, J., Xu, C., Xu, J., OuYang, B.
Nat. Commun. (2021), 12, 5106-5106, PubMed 34429434 , DOI 10.1038/s41467-021-25416-7 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 36293 released on 2020-09-22
Title membrane-bound PD-L1-CD

Related entities 1. PD-L1-TM monomer, : 10 entities Detail

Interaction partners 1. PD-L1-TM monomer, : 7 interactors Detail

More details for 7 interactors identified by 4 citations

Experiments performed 8 experiments Detail

1 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.5 mM 15N-13C-2H-PD-L1-TM, 30 mM DMPC, 60 mM DHPC

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.5 mM
2	MES	natural abundance		25 mM
3	DMPC	natural abundance		30 mM
4	DHPC	natural abundance		60 mM

2 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.5 mM 15N-13C-2H-PD-L1-TM, 30 mM DMPC, 60 mM DHPC

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.5 mM
2	MES	natural abundance		25 mM
3	DMPC	natural abundance		30 mM
4	DHPC	natural abundance		60 mM

3 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.5 mM 15N-13C-2H-PD-L1-TM, 30 mM DMPC, 60 mM DHPC

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.5 mM
2	MES	natural abundance		25 mM
3	DMPC	natural abundance		30 mM
4	DHPC	natural abundance		60 mM

4 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.5 mM 15N-13C-2H-PD-L1-TM, 30 mM DMPC, 60 mM DHPC

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.5 mM
2	MES	natural abundance		25 mM
3	DMPC	natural abundance		30 mM
4	DHPC	natural abundance		60 mM

5 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.5 mM 15N-13C-2H-PD-L1-TM, 30 mM DMPC, 60 mM DHPC

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.5 mM
2	MES	natural abundance		25 mM
3	DMPC	natural abundance		30 mM
4	DHPC	natural abundance		60 mM

6 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.5 mM 15N-13C-PD-L1-TM, 30 mM 2H-DMPC, 60 mM 2H-DHPC

#	Name	Isotope labeling	Type	Concentration
5	entity_1	[U-13C; U-15N]		0.5 mM
6	MES	natural abundance		25 mM
7	DMPC	[U-2H]		30 mM
8	DHPC	[U-2H]		60 mM

7 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.5 mM 15N-13C-PD-L1-TM, 30 mM 2H-DMPC, 60 mM 2H-DHPC

#	Name	Isotope labeling	Type	Concentration
5	entity_1	[U-13C; U-15N]		0.5 mM
6	MES	natural abundance		25 mM
7	DMPC	[U-2H]		30 mM
8	DHPC	[U-2H]		60 mM

8 2D 1H-13C HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.5 mM 15N-13C-PD-L1-TM, 30 mM 2H-DMPC, 60 mM 2H-DHPC

#	Name	Isotope labeling	Type	Concentration
5	entity_1	[U-13C; U-15N]		0.5 mM
6	MES	natural abundance		25 mM
7	DMPC	[U-2H]		30 mM
8	DHPC	[U-2H]		60 mM

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