BMRB: 51130

ASCC2 CUE domain interaction with monoubiquitin

Authors

Lombardi, P.M., Ngandu, C.K., Syed, S.H., Veilleux, J.M., Majumdar, A., Wolberger, C.

Assembly

ASCC2 CUE:monoubiquitin

Entity

1. ASCC2 CUE:monoubiquitin, entity 1 (polymer, Thiol state: all free), 55 monomers, 6331.075 Da Detail

ELDSLISQVK DLLPDLGEGF ILACLEYYHY DPEQVINNIL EERLAPTLSQ LDRNL

2. ASCC2 CUE:monoubiquitin, entity 2 (polymer, Thiol state: not present), 76 monomers, 8620.759 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGRQL EDGRTLSDYN IQRESTLHLV LRLRGG

Total weight

14951.834 Da

Max. entity weight

8620.759 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 38.9 %, Completeness: 10.7 %, Completeness (bb): 26.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	10.7 % (103 of 963)	6.5 % (53 of 816)	34.0 % (50 of 147)
Backbone	26.5 % (103 of 389)	20.1 % (53 of 264)	40.0 % (50 of 125)
Sidechain	0.0 % (0 of 574)	0.0 % (0 of 552)	0.0 % (0 of 22)
Aromatic	0.0 % (0 of 35)	0.0 % (0 of 35)
Methyl	0.0 % (0 of 88)	0.0 % (0 of 88)

1. entity 1

ELDSLISQVK DLLPDLGEGF ILACLEYYHY DPEQVINNIL EERLAPTLSQ LDRNL

2. entity 2

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGRQL EDGRTLSDYN IQRESTLHLV LRLRGG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 313 K, pH 7.0, Details in a buffer consisting of 20 mM Tris pH 7.0, 100 mM NaCl, and 200 mM TCEP

#	Name	Isotope labeling	Type	Concentration
1	ASCC2 CUE domain	[U-15N]		20 uM
2	monoubiquitin	natural abundance		681 uM
3	Tris	natural abundance		20 mM
4	NaCl	natural abundance		100 mM
5	TCEP	natural abundance		200 mM

Sample #2

Solvent system 90% H2O/10% D2O, Temperature 313 K, pH 7.0, Details in a buffer consisting of 20 mM Tris pH 7.0, 100 mM NaCl, and 200 mM TCEP

#	Name	Isotope labeling	Type	Concentration
6	ASCC2 CUE domain	[U-15N]		20 uM
7	Tris	natural abundance		20 mM
8	NaCl	natural abundance		100 mM
9	TCEP	natural abundance		200 mM

Hide sample codition

Release date

2021-10-09

Citation

The ASCC2 CUE domain in the ALKBH3-ASCC DNA repair complex recognizes adjacent ubiquitins in K63-linked polyubiquitin
Lombardi, P.M., Haile, S., Rusanov, T., Rodell, R., Anoh, R., Baer, J.G., Burke, K.A., Gray, L.N., Hacker, A.R., Kebreau, K.R., Ngandu, C.K., Orland, H.A., Osei-Asante, E., Schmelyun, D.P., Shorb, D.E., Syed, S.H., Veilleux, J.M., Majumdar, A., Mosammaparast, N., Wolberger, C.
J. Biol. Chem. (2022), 298, 101545-101545, PubMed 34971705 , DOI 10.1016/j.jbc.2021.101545 , Abstract

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. ASCC2 CUE:monoubiquitin, entity 1, : 1 : 2 : 12 entities Detail

Related entities 2. ASCC2 CUE:monoubiquitin, entity 2, : 98 : 223 entities Detail

Interaction partners 1. ASCC2 CUE:monoubiquitin, entity 1, : 49 interactors Detail

Experiments performed 1 experiments Detail