Backbone 1HN and 15N Chemical Shift Assignments for Sso7d I30V
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 25.4 % (124 of 488) | 14.7 % (62 of 421) | 92.5 % (62 of 67) |
Backbone | 61.9 % (122 of 197) | 45.5 % (61 of 134) | 96.8 % (61 of 63) |
Sidechain | 0.7 % (2 of 291) | 0.3 % (1 of 287) | 25.0 % (1 of 4) |
Aromatic | 8.0 % (2 of 25) | 4.2 % (1 of 24) | 100.0 % (1 of 1) |
Methyl | 0.0 % (0 of 28) | 0.0 % (0 of 28) |
1. Sso7d
MATVKFKYKG EEKQVDISKI KKVWRVGKMV SFTYDEGGGK TGRGAVSEKD APKELLQMLE KQKKTemperature 303 (±1) K, pH 5.07 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sso7d | [U-98% 15N] | 1.28 mM | |
2 | H2O | 90 % | ||
3 | D2O | 10 % | ||
4 | DSS | 100 uM |