BMRBj - BMREntryMaster

Found 1 Document (1.984 seconds)

BMRB: 6592

3UI2

Chromo 2 domain of cpSRP43

Authors

Vaithiyalingam, S., Thallapuranam, S.KRISHNASWAMY., Henry, R., Yu, C.

Assembly

Chromo2 domain of cpSRP43

Entity

1. Chromo2 domain of cpSRP43 (polymer, Thiol state: all free), 57 monomers, 6539.076 Da Detail

GSQVFEYAEV DEIVEKRGKG KDVEYLVRWK DGGDCEWVKG VHVAEDVAKD YEDGLEY

Formula weight

6539.076 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 84.5 %, Completeness (bb): 92.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	84.5 % (337 of 399)	84.1 % (285 of 339)	86.7 % (52 of 60)
Backbone	92.7 % (165 of 178)	93.4 % (113 of 121)	91.2 % (52 of 57)
Sidechain	77.8 % (172 of 221)	78.9 % (172 of 218)	0.0 % (0 of 3)
Aromatic	78.4 % (29 of 37)	82.9 % (29 of 35)	0.0 % (0 of 2)
Methyl	92.6 % (25 of 27)	92.6 % (25 of 27)

1. Chromo 2 domain

GSQVFEYAEV DEIVEKRGKG KDVEYLVRWK DGGDCEWVKG VHVAEDVAKD YEDGLEY

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD2	[U-15N; U-13C]		0.75 (±0.01) mM

Release date

2006-04-13

Citation

Three-dimensional solution structures of the chromodomains of cpSRP43
Sivaraja, V., Suresh Kumar, T.KRISHNASWAMY., Leena, P.SAGAYA-THERESA., Chang, A., Vidya, C., Goforth, R.L., Rajalingam, D., Arvind, K., Ye, J., Chou, J., Henry, R., Yu, C.
J. Biol. Chem. (2005), 280, 41465-41471, PubMed 16183644 , DOI 10.1074/jbc.M507077200 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

  BMRB: 6589 released on 2007-02-04
    Title Backbone 1H, 13C, and 15N Chemical Shift Assignments for Chromo Domain of cpSRP43
  BMRB: 6593 released on 2007-02-04
    Title Chromo 3 domain of cpSRP43
  Swiss-Prot: O22265 released on 2006-05-30
    Title SR43C_ARATH Entity Signal recognition particle 43 kDa protein, chloroplastic

Related entities 1. Chromo2 domain of cpSRP43, : 1 : 2 : 3 : 23 entities Detail

Interaction partners 1. Chromo2 domain of cpSRP43, : 21 interactors Detail

More details for 21 interactors identified by 5 citations

Experiments performed 9 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6592_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6592_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	Chromo-2_domain	Warning	382		96.5 (chain: 1, length: 57)
1	Chemical shift references	Chromo-2_domain_shifts	OK	3		No information

Assigned chemical shifts

Saveframe: Chromo-2_domain
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 57, Sequence coverage: 96.5%
- Total number of assignments
  - ¹H chemical shifts: 330
  - ¹⁵N chemical shifts: 52
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	339	280	82.6
¹⁵N chemical shifts	62	52	83.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	121	115	95.0
¹⁵N chemical shifts	57	52	91.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	218	165	75.7
¹⁵N chemical shifts	5	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	25	92.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	35	29	82.9
¹⁵N chemical shifts	2	0	0.0

Keywords cpSRP43, chromo 2 domain

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.984 seconds)

BMRB: 6592

Sample

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

Related entities 1. Chromo2 domain of cpSRP43, : 1 : 2 : 3 : 23 entities Detail

Interaction partners 1. Chromo2 domain of cpSRP43, : 21 interactors Detail

Experiments performed 9 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail