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BMRB: 6593

1X3P

Chromo 3 domain of cpSRP43

Authors

Philominathan, L.SAGAYA THERESA., Thallapuranam, S.KRISHNASWAMY., Henry, R., Yu, C.

Assembly

Chromo 3 domain of cpSRP43

Entity

1. Chromo 3 domain of cpSRP43 (polymer, Thiol state: not present), 54 monomers, 6113.701 Da Detail

AVAESVIGKR VGDDGKTIEY LVKWTDMSDA TWEPQDNVDS TLVLLYQQQQ PMNE

Formula weight

6113.701 Da

Source organism

Arabidopsis thaliana

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.7 %, Completeness: 70.9 %, Completeness (bb): 90.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	70.9 % (271 of 382)	70.1 % (225 of 321)	75.4 % (46 of 61)
Backbone	90.1 % (145 of 161)	90.8 % (99 of 109)	88.5 % (46 of 52)
Sidechain	57.0 % (126 of 221)	59.4 % (126 of 212)	0.0 % (0 of 9)
Aromatic	0.0 % (0 of 22)	0.0 % (0 of 20)	0.0 % (0 of 2)
Methyl	83.9 % (26 of 31)	83.9 % (26 of 31)

1. Chromo 3 domain of cpSRP43

AVAESVIGKR VGDDGKTIEY LVKWTDMSDA TWEPQDNVDS TLVLLYQQQQ PMNE

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD3	[U-15N; U-13C]	protein	0.75 (±0.05) mM

Release date

2007-02-04

Citation

Three-dimensional solution structures of the chromodomains of cpSRP43
Sivaraja, V., Suresh Kumar, T.KRISHNASWAMY., Leena, P.SAGAYA-THERESA., Chang, A., Vidya, C., Goforth, R.L., Rajalingam, D., Arvind, K., Ye, J., Chou, J., Henry, R., Yu, C.
J. Biol. Chem. (2005), 280, 41465-41471, PubMed 16183644 , DOI 10.1074/jbc.M507077200 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

  BMRB: 6589 released on 2007-02-04
    Title Backbone 1H, 13C, and 15N Chemical Shift Assignments for Chromo Domain of cpSRP43
  BMRB: 6592 released on 2006-04-13
    Title Chromo 2 domain of cpSRP43
  Swiss-Prot: O22265 released on 2006-05-30
    Title SR43C_ARATH Entity Signal recognition particle 43 kDa protein, chloroplastic

Related entities 1. Chromo 3 domain of cpSRP43, : 1 : 3 : 2 : 45 entities Detail

Interaction partners 1. Chromo 3 domain of cpSRP43, : 21 interactors Detail

More details for 21 interactors identified by 5 citations

Experiments performed 9 experiments Detail

1 2D 1H TOCSY

Instrument

Bruker Avance DMX - 700 MHz 700 MHz equipped with a cryoprobe

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD3	[U-15N; U-13C]	protein	0.75 (±0.05) mM

2 2D 1H NOESY

Instrument

Bruker Avance DMX - 700 MHz 700 MHz equipped with a cryoprobe

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD3	[U-15N; U-13C]	protein	0.75 (±0.05) mM

3 15N NOESY HSQC

Instrument

Bruker Avance DMX - 700 MHz 700 MHz equipped with a cryoprobe

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD3	[U-15N; U-13C]	protein	0.75 (±0.05) mM

4 15N HSQC DIPSY

Instrument

Bruker Avance DMX - 700 MHz 700 MHz equipped with a cryoprobe

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD3	[U-15N; U-13C]	protein	0.75 (±0.05) mM

5 HNCA

Instrument

Bruker Avance DMX - 700 MHz 700 MHz equipped with a cryoprobe

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD3	[U-15N; U-13C]	protein	0.75 (±0.05) mM

6 HNCOCA

Instrument

Bruker Avance DMX - 700 MHz 700 MHz equipped with a cryoprobe

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD3	[U-15N; U-13C]	protein	0.75 (±0.05) mM

7 CBCACONH

Instrument

Bruker Avance DMX - 700 MHz 700 MHz equipped with a cryoprobe

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD3	[U-15N; U-13C]	protein	0.75 (±0.05) mM

8 HNCACB

Instrument

Bruker Avance DMX - 700 MHz 700 MHz equipped with a cryoprobe

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD3	[U-15N; U-13C]	protein	0.75 (±0.05) mM

9 1H-15N HSQC

Instrument

Bruker Avance DMX - 700 MHz 700 MHz equipped with a cryoprobe

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD3	[U-15N; U-13C]	protein	0.75 (±0.05) mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr6593_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr6593_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	Chemical_Shift_list	Warning	316		90.7 (chain: 1, length: 54)
1	Chemical shift references	CD3_chemical_shifts	OK	3		No information

Assigned chemical shifts

Saveframe: Chemical_Shift_list
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 54, Sequence coverage: 90.7%
- Total number of assignments
  - ¹⁵N chemical shifts: 46
  - ¹H chemical shifts: 270
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords CD3, cpSRP43, chromo3 domain, Chromo 3 domain, structure