BMRBj - BMREntryMaster

Found 1 Document (1.132 seconds)

BMRB: 7153

1T8F

NMR spectroscopy of T4 Lysozyme peptide fragments

Authors

Najbar, L.V., Craik, D.J., Wade, J.D., McLeish, M.J.

Assembly

Lysozyme Loop

Entity

1. Lysozyme Loop (polymer, Thiol state: not present), 15 monomers, 1615.786 Da Detail

KAIGRNTNGV ITKDE

Formula weight

1615.786 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 80.8 %, Completeness (bb): 66.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	80.8 % (84 of 104)	95.4 % (83 of 87)	5.9 % (1 of 17)
Backbone	66.0 % (31 of 47)	93.8 % (30 of 32)	6.7 % (1 of 15)
Sidechain	93.0 % (53 of 57)	96.4 % (53 of 55)	0.0 % (0 of 2)
Methyl	88.9 % (8 of 9)	88.9 % (8 of 9)

1. loop (lys 48-62)

KAIGRNTNGV ITKDE

Show sample condition

Sample

Temperature 285 (±0) K

#	Name	Concentration
1	loop (lys 48-62)	1.0 ~ 2.0 mM
2	H2O	90 %
3	D2O	10 %

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 77.9 %, Completeness (bb): 64.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	77.9 % (162 of 208)	92.5 % (161 of 174)	2.9 % (1 of 34)
Backbone	64.9 % (61 of 94)	93.8 % (60 of 64)	3.3 % (1 of 30)
Sidechain	88.6 % (101 of 114)	91.8 % (101 of 110)	0.0 % (0 of 4)
Methyl	88.9 % (16 of 18)	88.9 % (16 of 18)

1. loop (lys 48-62)

KAIGRNTNGV ITKDE

Show sample condition

Sample

Temperature 285 (±0) K

#	Name	Concentration
4	loop (lys 48-62)	1.0 ~ 2.0 mM
5	TFE	50 %
6	H2O	40 %
7	D2O	10 %

Release date

2008-11-11

Citation

Identification of initiation sites for T4 lysozyme folding using CD and NMR spectroscopy of peptide fragments
Najbar, L.V., Craik, D.J., Wade, J.D., McLeish, M.J.
Biochemistry (2000), 39, 5911-5920, PubMed 10801343 , DOI 10.1021/bi000070i , Abstract

Entries sharing articles BMRB: 5 entries Detail

  BMRB: 7152 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7155 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7156 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7157 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7154 released on 2006-06-06
    Title NMR spectroscopy of T4 Lysozyme peptide fragments

Related entities 1. Lysozyme Loop, : 1 : 99 : 1 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7153_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7153_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	104	100.0 (chain: 1, length: 15)
2	Assigned chemical shifts	chem_shift_list_2	Warning	98	100.0 (chain: 1, length: 15)
1	Chemical shift references	chemical_shift_referencing	OK	1	No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 15, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 104
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: chem_shift_list_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 15, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 98
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
5	ARG	HH11	7.24
13	LYS	HZ1	7.24
13	LYS	HZ2	7.24
13	LYS	HZ3	7.24

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
5	ARG	HH11	7.23
13	LYS	HZ1	7.23
13	LYS	HZ2	7.23
13	LYS	HZ3	7.23

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Found 1 Document (1.132 seconds)

BMRB: 7153

Sample

Sample

Entries sharing articles BMRB: 5 entries Detail

Related entities 1. Lysozyme Loop, : 1 : 99 : 1 entities Detail

Experiments performed 1 experiments Detail

Instrument

Sample #1

Sample #2

Chemical shift validation 4 contents Detail