BMRBj - BMREntryMaster

Found 1 Document (0.667 seconds)

BMRB: 7155

1JQU

NMR spectroscopy of T4 Lysozyme peptide fragments

Authors

Najbar, L.V., Craik, D.J., Wade, J.D., McLeish, M.J.

Assembly

HELIX G

Entity

1. HELIX G (polymer, Thiol state: not present), 14 monomers, 1643.844 Da Detail

KRWDEAAVNL AKSR

Formula weight

1643.844 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 88.8 %, Completeness (bb): 92.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	88.8 % (79 of 89)	88.8 % (79 of 89)
Backbone	92.9 % (26 of 28)	92.9 % (26 of 28)
Sidechain	86.9 % (53 of 61)	86.9 % (53 of 61)
Aromatic	100.0 % (6 of 6)	100.0 % (6 of 6)
Methyl	100.0 % (7 of 7)	100.0 % (7 of 7)

1. helix G (lys 124-137)

KRWDEAAVNL AKSR

Show sample condition

Sample

Temperature 285 (±0) K

#	Name	Concentration
1	helix G (lys 124-137)	1.0 ~ 2.0 mM
2	H2O	90 %
3	D2O	10 %

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 91.0 %, Completeness (bb): 94.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	91.0 % (162 of 178)	91.0 % (162 of 178)
Backbone	94.6 % (53 of 56)	94.6 % (53 of 56)
Sidechain	89.3 % (109 of 122)	89.3 % (109 of 122)
Aromatic	100.0 % (12 of 12)	100.0 % (12 of 12)
Methyl	100.0 % (14 of 14)	100.0 % (14 of 14)

1. helix G (lys 124-137)

KRWDEAAVNL AKSR

Show sample condition

Sample

Temperature 303 (±0) K

#	Name	Concentration
4	helix G (lys 124-137)	1.0 ~ 2.0 mM
5	H2O	90 %
6	D2O	10 %
7	SDS	200 mM

Release date

2008-11-11

Citation

Identification of initiation sites for T4 lysozyme folding using CD and NMR spectroscopy of peptide fragments
Najbar, L.V., Craik, D.J., Wade, J.D., McLeish, M.J.
Biochemistry (2000), 39, 5911-5920, PubMed 10801343 , DOI: , Abstract

Entries sharing articles BMRB: 5 entries Detail

  BMRB: 7152 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7153 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7156 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7157 released on 2008-11-11
    Title NMR spectroscopy of T4 Lysozyme peptide fragments
  BMRB: 7154 released on 2006-06-06
    Title NMR spectroscopy of T4 Lysozyme peptide fragments

Related entities 1. HELIX G, : 1 : 99 : 1 entities Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7155_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7155_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	104	100.0 (chain: 1, length: 14)
2	Assigned chemical shifts	chem_shift_list_2	Warning	105	100.0 (chain: 1, length: 14)
1	Chemical shift references	chemical_shift_referencing	OK	1	No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 14, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 104
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: chem_shift_list_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 14, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 105
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
1	LYS	HZ1	7.36
1	LYS	HZ2	7.36
1	LYS	HZ3	7.36
2	ARG	HH11	7.2
12	LYS	HZ1	7.37
12	LYS	HZ2	7.37
12	LYS	HZ3	7.37
14	ARG	HH11	7.2

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
1	LYS	HZ1	7.47
1	LYS	HZ2	7.47
1	LYS	HZ3	7.47
2	ARG	HH11	6.98
12	LYS	HZ1	7.47
12	LYS	HZ2	7.47
12	LYS	HZ3	7.47
14	ARG	HH11	7.2

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Found 1 Document (0.667 seconds)

BMRB: 7155

Sample

Sample

Entries sharing articles BMRB: 5 entries Detail

Related entities 1. HELIX G, : 1 : 99 : 1 entities Detail

Chemical shift validation 4 contents Detail