Backbone 1HN, 15N, and 13C Chemical Shift Assignments for wt Im7* (* denotes his-tag) and its variants, Im7*L53AI54A and Im7*YY
MEHHHHHHEL KNSISDYTEA EFVQLLKEIE KENVAATDDV LDVLLEHFVK ITEHPDGGGG GYYPSDNRDD SPEGIVKEIK EWRAANGKPG FKQG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 37.5 % (405 of 1081) | 22.4 % (126 of 563) | 50.1 % (211 of 421) | 70.1 % (68 of 97) |
Backbone | 68.7 % (382 of 556) | 56.0 % (108 of 193) | 75.5 % (206 of 273) | 75.6 % (68 of 90) |
Sidechain | 13.9 % (85 of 610) | 4.9 % (18 of 370) | 28.8 % (67 of 233) | 0.0 % (0 of 7) |
Aromatic | 0.0 % (0 of 98) | 0.0 % (0 of 49) | 0.0 % (0 of 48) | 0.0 % (0 of 1) |
Methyl | 15.5 % (13 of 84) | 14.3 % (6 of 42) | 16.7 % (7 of 42) |
1. YY
MEHHHHHHEL KNSISDYTEA EFVQLLKEIE KENVAATDDV LDVLLEHFVK ITEHPDGGGG GYYPSDNRDD SPEGIVKEIK EWRAANGKPG FKQGTemperature 298 (±0.1) K, pH 7 (±0.2), Details doubly-labelled (13C/15N) Im7*YY sample for backbone resonance assignments.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Im7* | [U-13C; U-15N] | protein | 1 (±0.2) mM |
2 | potassium phosphate buffer | 50 mM | ||
3 | sodium sulphate | 400 mM | ||
4 | H2O | 90 % | ||
5 | D2O | 10 % |
Temperature 298 (±0.1) K, pH 7 (±0.2), Details singly-labelled (15N) Im7* sample for relaxation data measurement and 15N-edited NOESY experiment.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Im7* | [U-99% 15N] | protein | 1 (±0.2) mM |
7 | potassium phosphate buffer | 50 mM | ||
8 | sodium sulphate | 400 mM | ||
9 | H2O | 90 % | ||
10 | D2O | 10 % |