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BMRB: 11484


11484
2RV4
Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Authors
Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.
Assembly
protein
Entity
1. protein (polymer, Thiol state: all other bound), 37 monomers, 4244.794 Da Detail

GSSGSSGIKQ HCRFCKKKYS DVKNLIKHIR DMHDPQD


2. ZINC ION (non-polymer), 65.409 Da
Total weight
4310.203 Da
Max. entity weight
4244.794 Da
Entity Connection
na 4 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS12:SG2:ZN1:ZN
2nasing1:CYS15:SG2:ZN1:ZN
3nasing1:HIS28:NE22:ZN1:ZN
4nasing1:HIS33:NE22:ZN1:ZN

Source organism
Mus musculus
Exptl. method
solution NMR
Refine. method
DGSA-distance geometry simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 83.8 %, Completeness: 84.7 %, Completeness (bb): 78.2 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All84.7 % (372 of 439)84.7 % (199 of 235)86.1 % (142 of 165)79.5 % (31 of 39)
Backbone78.2 % (172 of 220)76.3 % (58 of 76)79.6 % (86 of 108)77.8 % (28 of 36)
Sidechain90.5 % (229 of 253)88.7 % (141 of 159)93.4 % (85 of 91)100.0 % (3 of 3)
Aromatic76.7 % (23 of 30)60.0 % (9 of 15)93.3 % (14 of 15)
Methyl100.0 % (20 of 20)100.0 % (10 of 10)100.0 % (10 of 10)

1. protein

GSSGSSGIKQ HCRFCKKKYS DVKNLIKHIR DMHDPQD

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0


#NameIsotope labelingTypeConcentration
1protein[U-13C; U-15N]protein1.09 mM
2TRIS[U-2H]20 mM
3sodium chloridenatural abundance100 mM
4DTT[U-2H]1 mM
5sodium azidenatural abundance0.02 %
6zinc chloridenatural abundance50 uM
7H2Osolvent90 %
8D2Osolvent10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2RV4, Strand ID: A Detail

Release date
2015-01-19
Citation
Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.
J. Struct. Funct. Genomics (2015), 16, 55-65, PubMed 25801860 , DOI 10.1007/s10969-015-9196-3 ,
Entries sharing articles BMRB: 15 entries Detail
  BMRB: 11474 released on 2015-01-19
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11475 released on 2015-01-19
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11476 released on 2015-01-19
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11477 released on 2015-01-19
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11478 released on 2015-01-19
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11479 released on 2015-01-19
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11480 released on 2015-01-19
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11481 released on 2015-01-19
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11482 released on 2015-01-19
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11483 released on 2015-01-19
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11485 released on 2015-01-19
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11486 released on 2013-03-11
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11487 released on 2013-03-11
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 11488 released on 2013-03-11
    Title Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
  BMRB: 10257 released on 2009-11-23
    Title Solution structures of the C2H2 type zinc finger domain of human zinc finger BED domain containing protein 2
Related entities 1. protein, : 1 : 1 : 5 : 47 entities Detail
More details for 54 related entities
Experiments performed 2 experiments Detail
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