Solution Structure of F153W cardiac troponin C
MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEWLEFMKGV E
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.1 % (1506 of 1858) | 83.4 % (809 of 970) | 75.3 % (541 of 718) | 91.8 % (156 of 170) |
Backbone | 83.8 % (806 of 962) | 95.5 % (317 of 332) | 71.3 % (336 of 471) | 96.2 % (153 of 159) |
Sidechain | 80.9 % (845 of 1045) | 77.1 % (492 of 638) | 88.4 % (350 of 396) | 27.3 % (3 of 11) |
Aromatic | 43.1 % (50 of 116) | 44.8 % (26 of 58) | 40.4 % (23 of 57) | 100.0 % (1 of 1) |
Methyl | 97.3 % (144 of 148) | 97.3 % (72 of 74) | 97.3 % (72 of 74) |
1. F153W
MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEWLEFMKGV ESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.5) K, pH 6.7 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | F153W | natural abundance | 1.0 (±0.2) mM | |
2 | Calcium | natural abundance | 10 (±1.0) mM | |
3 | potassium chloride | natural abundance | 100 (±10.0) mM |