BMRB: 17679

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Just Another Zinc Finger Protein residues 168-227

Authors

Burge, R.G., Martinez-Yamout, M.A., Dyson, H.JANE., Wright, P.E.

Assembly

JAZ ZF3

Entity

1. JAZ ZF3 (polymer, Thiol state: all other bound), 60 monomers, 7015.022 Da Detail

STKVEALHQN REMIDPDKFC SLCHATFNDP VMAQQHYVGK KHRKQETKLK LMARYGRLAD

2. ZN (non-polymer), 65.409 Da

Total weight

7080.431 Da

Max. entity weight

7015.022 Da

Entity Connection

na 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS20:SG	2:ZN1:ZN
2	na	sing	1:CYS23:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 32.0 %, Completeness (bb): 64.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	32.0 % (232 of 724)	15.4 % (59 of 383)	42.2 % (117 of 277)	87.5 % (56 of 64)
Backbone	64.6 % (230 of 356)	47.5 % (57 of 120)	65.7 % (117 of 178)	96.6 % (56 of 58)
Sidechain	1.4 % (6 of 426)	0.8 % (2 of 263)	2.5 % (4 of 157)	0.0 % (0 of 6)
Aromatic	0.0 % (0 of 52)	0.0 % (0 of 26)	0.0 % (0 of 26)
Methyl	0.0 % (0 of 52)	0.0 % (0 of 26)	0.0 % (0 of 26)

1. JAZ ZF3

STKVEALHQN REMIDPDKFC SLCHATFNDP VMAQQHYVGK KHRKQETKLK LMARYGRLAD

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	JAZ ZF3	[U-98% 13C; U-98% 15N]		300 uM
2	TRIS	natural abundance		25 mM
3	potassium chloride	natural abundance		50 mM
4	DTT	natural abundance		2 mM
5	zinc	natural abundance		50 uM
6	H2O	natural abundance		93 %
7	D2O	natural abundance		7 %

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Protein Blocks Logo

Calculated from 1 models in PDB: 2MKN, Strand ID: A Detail

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Release date

2014-02-23

Citation

Structural characterization of interactions between the double-stranded RNA-binding zinc finger protein JAZ and nucleic acids
Burge, R.G., Martinez-Yamout, M.A., Dyson, H., Wright, P.E.
Biochemistry (2014), 53, 1495-1510, PubMed 24521053 , DOI 10.1021/bi401675h , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

Related entities 1. JAZ ZF3, : 1 : 3 : 1 : 7 entities Detail

Interaction partners 1. JAZ ZF3, : 16 interactors Detail

Experiments performed 5 experiments Detail