BMRB: 25092

truncated EcMazE-DNA complex

Authors

Zorzini, V., Buts, L., Loris, R., van Nuland, N.

Assembly

MazE-DNA binding

Entity

1. MazE-DNA binding, entity 1 (polymer, Thiol state: not present), 67 monomers, 7658.564 × 2 Da Detail

NHKVHHHHHH MSDDDDKGIH SSVKRWGNSP AVRIPATLMQ ALNLNIDDEV KIDLVDGKLI IEPVRKE

2. DNA (5'-D(*CP*GP*TP*GP*AP*TP*AP*TP*AP*TP*AP*GP*TP*GP*C)-3') (polymer), 15 monomers, 4686.992 Da Detail

CGTGATATAT AGTGC

3. DNA (5'-D(P*GP*CP*AP*CP*TP*AP*TP*AP*TP*AP*TP*CP*AP*CP*G)-3') (polymer), 15 monomers, 4615.958 Da Detail

GCACTATATA TCACG

Total weight

24620.078 Da

Max. entity weight

7658.564 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 56.7 %, Completeness: 17.4 %, Completeness (bb): 26.7 % Detail

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Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	1H	15N
All	17.4 % (134 of 768)	10.7 % (75 of 698)	84.3 % (59 of 70)
Backbone	26.7 % (109 of 408)	16.3 % (56 of 344)	82.8 % (53 of 64)
Sidechain	6.9 % (25 of 360)	5.4 % (19 of 354)	100.0 % (6 of 6)
Aromatic	2.4 % (2 of 83)	1.2 % (1 of 82)	100.0 % (1 of 1)
Methyl	4.3 % (2 of 47)	4.3 % (2 of 47)

1. entity 1

NHKVHHHHHH MSDDDDKGIH SSVKRWGNSP AVRIPATLMQ ALNLNIDDEV KIDLVDGKLI IEPVRKE

2. DNA (5'-D(*CP*GP*TP*GP*AP*TP*AP*TP*AP*TP*AP*GP*TP*GP*C)-3')

CGTGATATAT AGTGC

3. DNA (5'-D(P*GP*CP*AP*CP*TP*AP*TP*AP*TP*AP*TP*CP*AP*CP*G)-3')

GCACTATATA TCACG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]		0.33 ~ 0.4 mM
2	DNA (5'-D(*CP*GP*TP*GP*AP*TP*AP*TP*AP*TP*AP*GP*TP*GP*C)-3')	natural abundance		0.0 ~ 0.4 mM
3	DNA (5'-D(P*GP*CP*AP*CP*TP*AP*TP*AP*TP*AP*TP*CP*AP*CP*G)-3')	natural abundance		0.0 ~ 0.4 mM
4	potassium phosphate	natural abundance		50 mM
5	sodium chloride	natural abundance		50 mM
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

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Protein Blocks Logo

Calculated from 7 models in PDB: 2MRU, Strand ID: A, B Detail

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Release date

2015-02-01

Citation

Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding
Zorzini, V., Buts, L., Schrank, E., Sterckx, Y.GJ., Respondek, M., Engelberg-Kulka, H., Loris, R., Zangger, K., van Nuland, N.AJ.
Nucleic Acids Res. (2015), 43, 1241-1256, PubMed 25564525 , DOI 10.1093/nar/gku1352 , Abstract

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. MazE-DNA binding, entity 1, : 1 : 1 entities Detail

Experiments performed 1 experiments Detail