BMRB: 25414

Structural features of a 3' splice site influenza A: 11-nt hairpin

Authors

Turner, D.H., Kennedy, S.D., Chen, J.L.

Assembly

3' splice site influenza A: 11-nt hairpin

Entity

1. 3' splice site influenza A: 11-nt hairpin (polymer, Thiol state: not present), 11 monomers, 3615.206 Da Detail

CCAGAAACGG A

Formula weight

3615.206 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.1 %, Completeness (bb): 97.4 % Detail

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Polymer type: polyribonucleotide

	Total	1H	31P
All	98.1 % (103 of 105)	98.9 % (93 of 94)	90.9 % (10 of 11)
Suger, PO4	97.4 % (75 of 77)	98.5 % (65 of 66)	90.9 % (10 of 11)
Nucleobase	100.0 % (28 of 28)	100.0 % (28 of 28)
Aromatic	100.0 % (22 of 22)	100.0 % (22 of 22)

1. RNA (11-MER)

CCAGAAACGG A

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.0, Details 11-nt hairpin

#	Name	Isotope labeling	Type	Concentration
1	entity	natural abundance		1.0 mM
2	H2O	natural abundance		95 %
3	D2O	natural abundance		5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.0, Details 11-nt hairpin

#	Name	Isotope labeling	Type	Concentration
4	entity	natural abundance		4.0 mM
5	D2O	natural abundance		100 %

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Release date

2015-05-03

Citation

Structural features of a 3' splice site in influenza a
Chen, J.L., Kennedy, S.D., Turner, D.H.
Biochemistry (2015), 54, 3269-3285, PubMed 25909229 , DOI 10.1021/acs.biochem.5b00012 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Experiments performed 7 experiments Detail