BMRBj - BMREntryMaster

Found 1 Document (1.017 seconds)

BMRB: 30608

6OSW

An order-to-disorder structural switch activates the FoxM1 transcription factor

Authors

Marceau, A.H., Rubin, S.M.

Assembly

Forkhead box M1

Entity

1. Forkhead box M1, entity 1 (polymer, Thiol state: not present), 94 monomers, 10498.98 Da Detail

GEFMRESPRR PIILKRRKLP FAKSTARSFP DGIRVMDHPT MPDTQVVVIP KSADLQSVIS VLTAKGKEAG PQGRNKFILL SGDTSAEEEN LYFQ

2. Forkhead box M1, entity 2 (polymer, Thiol state: not present), 56 monomers, 5947.530 Da Detail

GAQAGAANRS LTEGFVLDTM NDSLSKILVD ISFSGLEDED LGMGNISWSQ FIPEAK

Total weight

16446.51 Da

Max. entity weight

10498.98 Da

Source organism

Danio rerio

Exptl. method

solution NMR

Refine. method

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 56.0 %, Completeness: 23.2 %, Completeness (bb): 41.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	23.2 % (400 of 1724)	12.8 % (115 of 901)	31.2 % (209 of 670)	49.7 % (76 of 153)
Backbone	41.0 % (362 of 882)	28.4 % (86 of 303)	45.7 % (200 of 438)	53.9 % (76 of 141)
Sidechain	10.3 % (101 of 980)	4.8 % (29 of 598)	19.5 % (72 of 370)	0.0 % (0 of 12)
Aromatic	5.8 % (6 of 104)	11.5 % (6 of 52)	0.0 % (0 of 51)	0.0 % (0 of 1)
Methyl	8.7 % (14 of 160)	5.0 % (4 of 80)	12.5 % (10 of 80)

1. entity 1

GEFMRESPRR PIILKRRKLP FAKSTARSFP DGIRVMDHPT MPDTQVVVIP KSADLQSVIS VLTAKGKEAG PQGRNKFILL SGDTSAEEEN LYFQ

2. entity 2

GAQAGAANRS LTEGFVLDTM NDSLSKILVD ISFSGLEDED LGMGNISWSQ FIPEAK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details 270 uM [U-13C; U-15N; U-2H] FoxM1 NRD, 270 uM [U-13C; U-15N; U-2H] FoxM1 TAD, 20 mM sodium phosphate, 100 mM potassium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	FoxM1 NRD	[U-13C; U-15N; U-2H]	270 uM
2	FoxM1 TAD	[U-13C; U-15N; U-2H]	270 uM
3	sodium phosphate	natural abundance	20 mM
4	potassium chloride	natural abundance	100 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details 270 uM [U-13C; U-15N] FoxM1 NRD, 270 uM [U-13C] FoxM1 TAD, 20 mM sodium phosphate, 100 mM potassium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
5	FoxM1 NRD	[U-13C; U-15N]	270 uM
6	FoxM1 TAD	[U-13C]	270 uM
7	sodium phosphate	natural abundance	20 mM
8	potassium chloride	natural abundance	100 mM

Sample #3

#	Name	Isotope labeling	Concentration
9	FoxM1 NRD	[U-13C; U-15N; U-2H]	270 uM
10	FoxM1 TAD	[U-13C; U-15N; U-2H]	270 uM
11	sodium phosphate	natural abundance	20 mM
12	potassium chloride	natural abundance	100 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 6OSW, Strand ID: A, B Detail

Release date

2019-05-19

Citation

An order-to-disorder structural switch activates the FoxM1 transcription factor
Marceau, A.H., Brison, C., Nerli, S., Arsenault, H.E., McShane, A.C., Chen, E., Lee, H.W., Benanti, J.A., Sgourakis, N.G., Rubin, S.M.
Elife (2019), 8, e46131-e46131, PubMed 31134895 , DOI 10.7554/eLife.46131 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 27763 released on 2019-01-21
    Title FoxM1 Transactivation Domain
  BMRB: 27764 released on 2019-01-21
    Title FoxM1 Transactivation Domain, Phosphorylated form

Related entities 1. Forkhead box M1, entity 1, : 1 : 9 entities Detail