BMRBj - BMREntryMaster

Found 1 Document (0.945 seconds)

BMRB: 27763

FoxM1 Transactivation Domain

Authors

Marceau, A.H., Brison, C., Nerli, S., Arsenault, H.E., McShane, A.C., Chen, E., Lee, H., Benanti, J.A., Sgourakis, N.G., Rubin, S.M.

Assembly

FoxM1 TAD

Entity

1. FoxM1 TAD (polymer, Thiol state: not present), 55 monomers, 5995.613 Da Detail

GEFGAANRSL TEGFVLDTMN DSLSKILVDI SFSGLEDEDL GMGNISWSQF IPELK

Formula weight

5995.613 Da

Source organism

Danio rerio

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 45.7 %, Completeness (bb): 74.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.7 % (284 of 621)	15.7 % (50 of 319)	76.1 % (185 of 243)	83.1 % (49 of 59)
Backbone	74.1 % (243 of 328)	42.6 % (49 of 115)	91.2 % (145 of 159)	90.7 % (49 of 54)
Sidechain	25.7 % (88 of 342)	0.5 % (1 of 204)	65.4 % (87 of 133)	0.0 % (0 of 5)
Aromatic	1.9 % (1 of 52)	3.8 % (1 of 26)	0.0 % (0 of 25)	0.0 % (0 of 1)
Methyl	40.0 % (24 of 60)	0.0 % (0 of 30)	80.0 % (24 of 30)

1. FoxM1 TAD

GEFGAANRSL TEGFVLDTMN DSLSKILVDI SFSGLEDEDL GMGNISWSQF IPELK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	FoxM1 TAD	[U-100% 13C; U-100% 15N]	450 mM
2	D2O	[U-2H]	10 %
3	sodium chloride	natural abundance	100 mM
4	sodium phosphate	natural abundance	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 1 Detail

Release date

2019-01-21

Citation

An order-to-disorder structural switch activates the FoxM1 transcription factor
Marceau, A.H., Brison, C., Nerli, S., Arsenault, H.E., McShane, A.C., Chen, E., Lee, H., Benanti, J.A., Sgourakis, N.G., Rubin, S.M.
Elife (2019), 8, e46131-e46131, PubMed 31134895 , DOI 10.7554/eLife.46131 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 30608 released on 2019-05-19
    Title An order-to-disorder structural switch activates the FoxM1 transcription factor
  BMRB: 27764 released on 2019-01-21
    Title FoxM1 Transactivation Domain, Phosphorylated form

Related entities 1. FoxM1 TAD, : 9 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr27763_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr27763_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	284		94.5 (chain: 1, length: 55)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 55, Sequence coverage: 94.5%
- Total number of assignments
  - ¹³C chemical shifts: 185
  - ¹H chemical shifts: 50
  - ¹⁵N chemical shifts: 49
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	243	185	76.1
¹H chemical shifts	319	50	15.7
¹⁵N chemical shifts	60	49	81.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	110	98	89.1
¹H chemical shifts	115	49	42.6
¹⁵N chemical shifts	54	49	90.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	133	87	65.4
¹H chemical shifts	204	1	0.5
¹⁵N chemical shifts	6	0	0.0

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Found 1 Document (0.945 seconds)

BMRB: 27763

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. FoxM1 TAD, : 9 entities Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail