An order-to-disorder structural switch activates the FoxM1 transcription factor
GEFMRESPRR PIILKRRKLP FAKSTARSFP DGIRVMDHPT MPDTQVVVIP KSADLQSVIS VLTAKGKEAG PQGRNKFILL SGDTSAEEEN LYFQ
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 23.2 % (400 of 1724) | 12.8 % (115 of 901) | 31.2 % (209 of 670) | 49.7 % (76 of 153) |
Backbone | 41.0 % (362 of 882) | 28.4 % (86 of 303) | 45.7 % (200 of 438) | 53.9 % (76 of 141) |
Sidechain | 10.3 % (101 of 980) | 4.8 % (29 of 598) | 19.5 % (72 of 370) | 0.0 % (0 of 12) |
Aromatic | 5.8 % (6 of 104) | 11.5 % (6 of 52) | 0.0 % (0 of 51) | 0.0 % (0 of 1) |
Methyl | 8.7 % (14 of 160) | 5.0 % (4 of 80) | 12.5 % (10 of 80) |
1. entity 1
GEFMRESPRR PIILKRRKLP FAKSTARSFP DGIRVMDHPT MPDTQVVVIP KSADLQSVIS VLTAKGKEAG PQGRNKFILL SGDTSAEEEN LYFQ2. entity 2
GAQAGAANRS LTEGFVLDTM NDSLSKILVD ISFSGLEDED LGMGNISWSQ FIPEAKSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details 270 uM [U-13C; U-15N; U-2H] FoxM1 NRD, 270 uM [U-13C; U-15N; U-2H] FoxM1 TAD, 20 mM sodium phosphate, 100 mM potassium chloride, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FoxM1 NRD | [U-13C; U-15N; U-2H] | 270 uM | |
2 | FoxM1 TAD | [U-13C; U-15N; U-2H] | 270 uM | |
3 | sodium phosphate | natural abundance | 20 mM | |
4 | potassium chloride | natural abundance | 100 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details 270 uM [U-13C; U-15N] FoxM1 NRD, 270 uM [U-13C] FoxM1 TAD, 20 mM sodium phosphate, 100 mM potassium chloride, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | FoxM1 NRD | [U-13C; U-15N] | 270 uM | |
6 | FoxM1 TAD | [U-13C] | 270 uM | |
7 | sodium phosphate | natural abundance | 20 mM | |
8 | potassium chloride | natural abundance | 100 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details 270 uM [U-13C; U-15N; U-2H] FoxM1 NRD, 270 uM [U-13C; U-15N; U-2H] FoxM1 TAD, 20 mM sodium phosphate, 100 mM potassium chloride, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | FoxM1 NRD | [U-13C; U-15N; U-2H] | 270 uM | |
10 | FoxM1 TAD | [U-13C; U-15N; U-2H] | 270 uM | |
11 | sodium phosphate | natural abundance | 20 mM | |
12 | potassium chloride | natural abundance | 100 mM |