BMRBj - BMREntryMaster

Found 1 Document (0.483 seconds)

BMRB: 27764

FoxM1 Transactivation Domain, Phosphorylated form

Authors

Marceau, A.H., Brison, C., Nerli, S., Arsenault, H.E., McShane, A.C., Chen, E., Lee, H., Benanti, J.A., Sgourakis, N.G., Rubin, S.M.

Assembly

Phosphorylated FoxM1 TAD

Entity

1. Phosphorylated FoxM1 TAD (polymer, Thiol state: not present), 55 monomers, 6315.529 Da Detail

GEFGAANRXL TEGFVLDTMN DXLSKILVDI XFSGLEDEDL GMGNISWXQF IPELK

Formula weight

6315.529 Da

Source organism

Danio rerio

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 90.9 %, Completeness: 42.4 %, Completeness (bb): 75.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.4 % (250 of 589)	21.8 % (66 of 303)	59.7 % (138 of 231)	83.6 % (46 of 55)
Backbone	75.0 % (228 of 304)	46.7 % (50 of 107)	89.8 % (132 of 147)	92.0 % (46 of 50)
Sidechain	20.0 % (66 of 330)	8.2 % (16 of 196)	38.8 % (50 of 129)	0.0 % (0 of 5)
Aromatic	1.9 % (1 of 52)	3.8 % (1 of 26)	0.0 % (0 of 25)	0.0 % (0 of 1)
Methyl	15.0 % (9 of 60)	10.0 % (3 of 30)	20.0 % (6 of 30)

1. Phosphorylated FoxM1 TAD

GEFGAANRXL TEGFVLDTMN DXLSKILVDI XFSGLEDEDL GMGNISWXQF IPELK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	Phosphorylated FoxM1 TAD	[U-100% 13C; U-100% 15N]	450 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	10 mM

Chem. Shift Complete2

Sequence coverage: 12.7 %, Completeness: 23.8 %, Completeness (bb): 42.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	23.8 % (280 of 1178)	12.7 % (77 of 606)	32.7 % (151 of 462)	47.3 % (52 of 110)
Backbone	42.3 % (257 of 608)	28.0 % (60 of 214)	49.3 % (145 of 294)	52.0 % (52 of 100)
Sidechain	11.1 % (73 of 660)	4.3 % (17 of 392)	21.7 % (56 of 258)	0.0 % (0 of 10)
Aromatic	1.0 % (1 of 104)	1.9 % (1 of 52)	0.0 % (0 of 50)	0.0 % (0 of 2)
Methyl	9.2 % (11 of 120)	6.7 % (4 of 60)	11.7 % (7 of 60)

1. Phosphorylated FoxM1 TAD

GEFGAANRXL TEGFVLDTMN DXLSKILVDI XFSGLEDEDL GMGNISWXQF IPELK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	Phosphorylated FoxM1 TAD	[U-100% 13C; U-100% 15N]	450 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	10 mM

Chem. Shift Complete3

Sequence coverage: 14.5 %, Completeness: 18.3 %, Completeness (bb): 31.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	18.3 % (323 of 1767)	10.5 % (95 of 909)	24.8 % (172 of 693)	33.9 % (56 of 165)
Backbone	31.1 % (284 of 912)	21.5 % (69 of 321)	36.1 % (159 of 441)	37.3 % (56 of 150)
Sidechain	9.7 % (96 of 990)	4.4 % (26 of 588)	18.1 % (70 of 387)	0.0 % (0 of 15)
Aromatic	0.6 % (1 of 156)	1.3 % (1 of 78)	0.0 % (0 of 75)	0.0 % (0 of 3)
Methyl	11.7 % (21 of 180)	8.9 % (8 of 90)	14.4 % (13 of 90)

1. Phosphorylated FoxM1 TAD

GEFGAANRXL TEGFVLDTMN DXLSKILVDI XFSGLEDEDL GMGNISWXQF IPELK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	Phosphorylated FoxM1 TAD	[U-100% 13C; U-100% 15N]	450 mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	10 mM

Release date

2019-01-21

Citation

An order-to-disorder structural switch activates the FoxM1 transcription factor
Marceau, A.H., Brison, C., Nerli, S., Arsenault, H.E., McShane, A.C., Chen, E., Lee, H., Benanti, J.A., Sgourakis, N.G., Rubin, S.M.
Elife (2019), 8, e46131-e46131, PubMed 31134895 , DOI 10.7554/eLife.46131 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 30608 released on 2019-05-19
    Title An order-to-disorder structural switch activates the FoxM1 transcription factor
  BMRB: 27763 released on 2019-01-21
    Title FoxM1 Transactivation Domain

Related entities 1. Phosphorylated FoxM1 TAD, : 9 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 5 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr27764_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
1	9	SEP	PHOSPHOSERINE	None
1	22	SEP	PHOSPHOSERINE	Assigned chemical shifts
1	31	SEP	PHOSPHOSERINE	Assigned chemical shifts
1	48	SEP	PHOSPHOSERINE	Assigned chemical shifts

Content subtype: bmr27764_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	241	96.4 (chain: 1, length: 55)
2	Assigned chemical shifts	assigned_chemical_shifts_2	Warning	17	7.3 (chain: 1, length: 55)
3	Assigned chemical shifts	assigned_chemical_shifts_3	Warning	4	1.8 (chain: 1, length: 55)
1	Chemical shift references	chem_shift_reference_1	OK	3	No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 55, Sequence coverage: 96.4%
- Total number of assignments
  - ¹³C chemical shifts: 142
  - ¹H chemical shifts: 50
  - ¹⁵N chemical shifts: 49
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: assigned_chemical_shifts_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 55, Sequence coverage: 7.3%
- Total number of assignments
  - ¹H chemical shifts: 4
  - ¹³C chemical shifts: 9
  - ¹⁵N chemical shifts: 4
- Completeness of assignments
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: assigned_chemical_shifts_3
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 55, Sequence coverage: 1.8%
- Total number of assignments
  - ¹H chemical shifts: 1
  - ¹³C chemical shifts: 2
  - ¹⁵N chemical shifts: 1
- Completeness of assignments
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	239	139	58.2
¹H chemical shifts	319	50	15.7
¹⁵N chemical shifts	60	49	81.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	106	92	86.8
¹H chemical shifts	115	49	42.6
¹⁵N chemical shifts	54	49	90.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	133	47	35.3
¹H chemical shifts	204	1	0.5
¹⁵N chemical shifts	6	0	0.0

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Found 1 Document (0.483 seconds)

BMRB: 27764

Sample

Sample

Sample

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. Phosphorylated FoxM1 TAD, : 9 entities Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 5 contents Detail

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
22	SEP	C	174.525
31	SEP	C	174.059
48	SEP	C	174.998