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Found 1 Document (0.556 seconds)

BMRB: 34710

7YYI

Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) TMD

Authors

Silber, M., Muhle-Goll, C.

Assembly

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155)

Entity

1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155) (polymer, Thiol state: not present), 29 monomers, 3544.366 Da Detail

KKKLGFFLVT FGFIWGMMLL HFTIQQRKK

Formula weight

3544.366 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 72.3 %, Completeness (bb): 79.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.3 % (295 of 408)	76.6 % (164 of 214)	61.5 % (99 of 161)	97.0 % (32 of 33)
Backbone	79.9 % (139 of 174)	90.2 % (55 of 61)	65.5 % (55 of 84)	100.0 % (29 of 29)
Sidechain	70.0 % (182 of 260)	71.2 % (109 of 153)	68.0 % (70 of 103)	75.0 % (3 of 4)
Aromatic	45.5 % (30 of 66)	57.6 % (19 of 33)	31.3 % (10 of 32)	100.0 % (1 of 1)
Methyl	87.5 % (28 of 32)	87.5 % (14 of 16)	87.5 % (14 of 16)

1. entity 1

KKKLGFFLVT FGFIWGMMLL HFTIQQRKK

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 300 K, pH 7.0, Details 0.500 mM GNTV_WT, trifluoroethanol/water

#	Name	Isotope labeling	Type	Concentration
1	GNTV_WT	natural abundance		0.500 mM

Release date

2022-03-22

Citation

Helical stability of the GnTV transmembrane domain impacts on SPPL3 dependent cleavage
Papadopoulou, A.A., Stelzer, W., Silber, M., Schlosser, C., Spitz, C., Haug-Kroper, M., Straub, T., Muller, S.A., Lichtenthaler, S.F., Muhle-Goll, C., Langosch, D., Fluhrer, R.
Sci. Rep. (2022), 12, 20987-20987, PubMed 36470941 , DOI 10.1038/s41598-022-24772-8 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 34711 released on 2022-03-16
    Title Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) G26P mutant TMD
  BMRB: 34712 released on 2022-03-16
    Title Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) G22L mutant TMD
  BMRB: 34713 released on 2022-03-16
    Title Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) G22L and G26L double mutant TMD

Related entities 1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155), : 1 : 3 : 13 entities Detail

Interaction partners 1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155), : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34710_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34710_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	319		96.6 (chain: 1, length: 29)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 29, Sequence coverage: 96.6%
- Total number of assignments
  - ¹H chemical shifts: 195
  - ¹³C chemical shifts: 93
  - ¹⁵N chemical shifts: 31
- Completeness of assignments
  - Entity_assemble_ID: 1
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
15	TRP	CE2	120.084

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	214	167	78.0
¹³C chemical shifts	161	92	57.1
¹⁵N chemical shifts	33	31	93.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	61	58	95.1
¹³C chemical shifts	58	28	48.3
¹⁵N chemical shifts	29	28	96.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	153	109	71.2
¹³C chemical shifts	103	64	62.1
¹⁵N chemical shifts	4	3	75.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	18	14	77.8
¹³C chemical shifts	18	14	77.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	33	18	54.5
¹³C chemical shifts	32	8	25.0
¹⁵N chemical shifts	1	1	100.0

Keywords Glycosyltransferase, GnTV, Golgi, MEMBRANE PROTEIN, SPPL3, TMD, intramembrane

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Found 1 Document (0.556 seconds)

BMRB: 34710

Sample

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155), : 1 : 3 : 13 entities Detail

Interaction partners 1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155), : 2 interactors Detail

Experiments performed 4 experiments Detail

Instrument

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Instrument

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Instrument

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Chemical shift validation 3 contents Detail