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BMRB: 4452


4452
2AFP
The Solution structure of Type II Antifreeze Protein Reveals a New Member of the Lectin Family
Authors
Gronwald, W., Loewen, M.C., Lix, B., Daugulis, A.J., Sonnichsen, F.D., Davies, P.L., Sykes, B.D.
Assembly
sea raven Type II antifreeze protein
Entity
1. sea raven Type II antifreeze protein (polymer, Thiol state: all disulfide bound), 129 monomers, 13992.76 Da Detail

QRAGPNCPAG WQPLGDRCIY YETTAMTWAL AETNCMKLGG HLASIHSQEE HSFIQTLNAG VVWIGGSACL QAGAWTWSDG TPMNFRSWCS TKPDDVLAAC CMQMTAAADQ CWDDLPCPAS HKSVCAMTF


Formula weight
13992.76 Da
Entity Connection
disulfide 5 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS7:SG1:CYS18:SG
2disulfidesing1:CYS35:SG1:CYS125:SG
3disulfidesing1:CYS69:SG1:CYS100:SG
4disulfidesing1:CYS89:SG1:CYS111:SG
5disulfidesing1:CYS101:SG1:CYS117:SG

Source organism
Hemitripterus americanus
Exptl. method
NMR
Refine. method
SIMULATED ANNEALING
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 93.8 %, Completeness: 80.3 %, Completeness (bb): 96.1 % Detail
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Polymer type: polypeptide(L)

Total1H15N
All80.3 % (689 of 858)78.8 % (566 of 718)87.9 % (123 of 140)
Backbone96.1 % (368 of 383)95.4 % (249 of 261)97.5 % (119 of 122)
Sidechain67.6 % (321 of 475)69.4 % (317 of 457)22.2 % (4 of 18)
Aromatic72.5 % (58 of 80)78.1 % (57 of 73)14.3 % (1 of 7)
Methyl93.2 % (55 of 59)93.2 % (55 of 59)

1. sea raven Type II antifreeze protein

QRAGPNCPAG WQPLGDRCIY YETTAMTWAL AETNCMKLGG HLASIHSQEE HSFIQTLNAG VVWIGGSACL QAGAWTWSDG TPMNFRSWCS TKPDDVLAAC CMQMTAAADQ CWDDLPCPAS HKSVCAMTF

Show sample condition
Sample

Temperature 308 (±0.1) K, pH 5.6 (±0.1)


#NameIsotope labelingTypeConcentration
1sea raven Type II antifreeze protein[U-15N]0.5 ~ 1.2 mM

Protein Blocks Logo
Calculated from 5 models in PDB: 2AFP, Strand ID: A Detail

Release date
2000-01-11
Citation 1
The solution structure of type II antifreeze protein reveals a new member of the lectin family
Gronwald, W., Loewen, M.C., Lix, B., Daugulis, A.J., Sonnichsen, F.D., Davies, P.L., Sykes, B.D.
Biochemistry (1998), 37, 4712-4721, PubMed 9537986 , DOI 10.1021/bi972788c ,
Show more 2 citaions
Citation 2
Crystallographic R factor refinement by molecular dynamics
Brunger, Kuriyan, Karplus
Science (1987), 235, 458-460, PubMed 17810339 , DOI 10.1126/science.235.4787.458 ,
Citation 3
A short recollection on the paper entitled "A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams" by D.S. Garrett, R. Powers, A.M. Gronenborn, and G.M. Clore [J. Magn. Reson. 95 (1991) 214-220]
J. Magn. Reson. (2011), 213, 364-365, PubMed 22152356 , DOI 10.1016/j.jmr.2011.08.009 ,
Entries sharing articles BMRB: 8, Swiss-Prot: 1 entries Detail
  BMRB: 4401 released on 2005-05-25
    Title Structure and Interaction Site of the Regulatory Domain of Troponin-C when Complexed with the 96-148 Region of Troponin-I
  BMRB: 5854 released on 2004-02-09
    Title 1H, 13C, and 15N backbone assignments for the 60.8 kD dimer of the NAD+ synthetase from Bacillus subtilis
  BMRB: 5334 released on 2003-06-09
    Title Backbone 1H, 13C, and 15N resonance assignments and secondary structure of the D54A mutant of HTLV-I capsid protein
  BMRB: 5189 released on 2002-05-06
    Title 1H, 15N and 13C resonance assignments of rabbit apo-S100A11
  BMRB: 4981 released on 2001-04-30
    Title Identification of the phospholipid binding site of human beta2-glycoprotein I domain V by heteronuclear magnetic resonance
  BMRB: 4389 released on 2000-12-14
    Title Backbone NMR Assignment and Secondary Structure of Ribosome Recycling Factor (RRF) from Pseudomonas aeruginosa
  BMRB: 4250 released on 2000-02-24
    Title Structure of the 3' hairpin of the TYMV pseudoknot: Preformation in RNA folding
  BMRB: 4249 released on 1999-08-05
    Title Solution Structure of the DNA- and RPA-binding Domain of the Human Repair Factor XPA
  Swiss-Prot: P05140 released on 1987-08-13
    Title ISP2_HEMAM Entity Type-2 ice-structuring protein
Related entities 1. sea raven Type II antifreeze protein, : 1 : 1 : 289 entities Detail
More details for 291 related entities
Experiments performed 6 experiments Detail
nullKeywords recombinant sea raven protein, solution backbone fold