BMRBj - BMREntryMaster

Found 1 Document (0.577 seconds)

BMRB: 15010

2JVK

Backbone and Side Chain 1H, 13C and 15N Chemical Shift Assignments for Sporulation phosphotransferase F mutant L66A

Authors

McLaughlin, P.D., Bobay, B.G., Regel, E.J., Thompson, R.J., Hoch, J.A., Cavanagh, J.

Assembly

Spo0FL66A monomer

Entity

1. Spo0FL66A monomer (polymer, Thiol state: all free), 132 monomers, 15250.54 Da Detail

MMNEKILIVD DQYGIRILLN EVFNKEGYQT FQAANGLQAL DIVTKERPDL VLLDMKIPGM DGIEIAKRMK VIDENIRVII MTAYGELDMI QESKELGALT HFAKPFDIDE IRDAVKKYLP LKSNLEHHHH HH

Formula weight

15250.54 Da

Source organism

Bacillus subtilis

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 82.4 %, Completeness (bb): 90.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.4 % (1318 of 1599)	82.4 % (688 of 835)	80.2 % (501 of 625)	92.8 % (129 of 139)
Backbone	90.9 % (713 of 784)	95.1 % (254 of 267)	87.4 % (340 of 389)	93.0 % (119 of 128)
Sidechain	77.2 % (726 of 940)	76.4 % (434 of 568)	78.1 % (282 of 361)	90.9 % (10 of 11)
Aromatic	30.0 % (30 of 100)	42.0 % (21 of 50)	18.0 % (9 of 50)
Methyl	92.9 % (156 of 168)	96.4 % (81 of 84)	89.3 % (75 of 84)

1. Spo0FL66A

MMNEKILIVD DQYGIRILLN EVFNKEGYQT FQAANGLQAL DIVTKERPDL VLLDMKIPGM DGIEIAKRMK VIDENIRVII MTAYGELDMI QESKELGALT HFAKPFDIDE IRDAVKKYLP LKSNLEHHHH HH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	Spo0FL66A	[U-100% 13C; U-100% 15N]	1.0 ~ 2.0 mM
2	TRIS	none	25 mM
3	potassium chloride	none	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.74 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: 2.28 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.74 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: 2.28 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.74 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.74 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 4 Detail

Release date

2007-05-08

Citation

Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition
McLaughlin, P.D., Bobay, B.G., Regel, E.J., Thompson, R.J., Hoch, J.A., Cavanagh, J.
FEBS Lett. (2007), 581, 1425-1429, PubMed 17350627 , DOI 10.1016/j.febslet.2007.02.061 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 15008 released on 2007-05-08
    Title Backbone and Side Chain 1H, 13C and 15N Chemical Shift Assignments for Sporulation Phosphotransferase F Mutant H101A
  BMRB: 15009 released on 2007-05-08
    Title Backbone and Side Chain 1H, 13C, 15N Chemical Shift Assignments for Sporulation Phosphotransferase F Mutant I90A
  BMRB: 15011 released on 2007-05-08
    Title Backbone and Side Chain 1H, 13C and 15N Chemical Shift Assignments for Sporulation phosphotransferase F mutant Y13A

Related entities 1. Spo0FL66A monomer, : 1 : 12 : 272 entities Detail

Interaction partners 1. Spo0FL66A monomer, : 6 interactors Detail

More details for 6 interactors identified by 4 citations

Experiments performed 8 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15010_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15010_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1409		96.2 (chain: 1, length: 132)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 132, Sequence coverage: 96.2%
- Total number of assignments
  - ¹³C chemical shifts: 476
  - ¹H chemical shifts: 809
  - ¹⁵N chemical shifts: 124
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	625	476	76.2
¹H chemical shifts	835	649	77.7
¹⁵N chemical shifts	144	124	86.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	264	209	79.2
¹H chemical shifts	267	249	93.3
¹⁵N chemical shifts	128	116	90.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	361	267	74.0
¹H chemical shifts	568	400	70.4
¹⁵N chemical shifts	16	8	50.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	91	73	80.2
¹H chemical shifts	91	80	87.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	50	0	0.0
¹H chemical shifts	50	12	24.0

Keywords mutant, Spo0F, sporulation, surface recognition

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Found 1 Document (0.577 seconds)

BMRB: 15010

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. Spo0FL66A monomer, : 1 : 12 : 272 entities Detail

Interaction partners 1. Spo0FL66A monomer, : 6 interactors Detail

Experiments performed 8 experiments Detail

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Chemical shift validation 3 contents Detail