BMRBj - BMREntryMaster

Found 1 Document (0.67 seconds)

BMRB: 15011

2JVJ

Backbone and Side Chain 1H, 13C and 15N Chemical Shift Assignments for Sporulation phosphotransferase F mutant Y13A

Authors

McLaughlin, P.D., Bobay, B.G., Regel, E.J., Thompson, R.J., Hoch, J.A., Cavanagh, J.

Assembly

Spo0FY13A monomer

Entity

1. Spo0FY13A monomer (polymer, Thiol state: all free), 132 monomers, 15200.53 Da Detail

MMNEKILIVD DQAGIRILLN EVFNKEGYQT FQAANGLQAL DIVTKERPDL VLLDMKIPGM DGIEILKRMK VIDENIRVII MTAYGELDMI QESKELGALT HFAKPFDIDE IRDAVKKYLP LKSNLEHHHH HH

Formula weight

15200.53 Da

Source organism

Bacillus subtilis

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 87.2 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.2 % (1392 of 1597)	85.9 % (716 of 834)	87.8 % (548 of 624)	92.1 % (128 of 139)
Backbone	95.5 % (749 of 784)	95.5 % (255 of 267)	96.1 % (374 of 389)	93.8 % (120 of 128)
Sidechain	81.3 % (763 of 938)	81.3 % (461 of 567)	81.7 % (294 of 360)	72.7 % (8 of 11)
Aromatic	7.6 % (7 of 92)	15.2 % (7 of 46)	0.0 % (0 of 46)
Methyl	97.7 % (168 of 172)	97.7 % (84 of 86)	97.7 % (84 of 86)

1. Spo0FY13A

MMNEKILIVD DQAGIRILLN EVFNKEGYQT FQAANGLQAL DIVTKERPDL VLLDMKIPGM DGIEILKRMK VIDENIRVII MTAYGELDMI QESKELGALT HFAKPFDIDE IRDAVKKYLP LKSNLEHHHH HH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	Spo0FY13A	[U-99% 13C; U-99% 15N]	1.0 ~ 2.0 mM
2	TRIS	none	25 mM
3	potassium chloride	none	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.74 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	direct	0.1013291

Referencing offset: 2.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.74 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	direct	0.1013291

Referencing offset: 2.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.74 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	direct	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.74 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	internal	direct	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Release date

2007-05-08

Citation

Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition
McLaughlin, P.D., Bobay, B.G., Regel, E.J., Thompson, R.J., Hoch, J.A., Cavanagh, J.
FEBS Lett. (2007), 581, 1425-1429, PubMed 17350627 , DOI 10.1016/j.febslet.2007.02.061 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 15008 released on 2007-05-08
    Title Backbone and Side Chain 1H, 13C and 15N Chemical Shift Assignments for Sporulation Phosphotransferase F Mutant H101A
  BMRB: 15009 released on 2007-05-08
    Title Backbone and Side Chain 1H, 13C, 15N Chemical Shift Assignments for Sporulation Phosphotransferase F Mutant I90A
  BMRB: 15010 released on 2007-05-08
    Title Backbone and Side Chain 1H, 13C and 15N Chemical Shift Assignments for Sporulation phosphotransferase F mutant L66A

Related entities 1. Spo0FY13A monomer, : 1 : 8 : 275 entities Detail

Interaction partners 1. Spo0FY13A monomer, : 6 interactors Detail

More details for 6 interactors identified by 4 citations

Experiments performed 8 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15011_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15011_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1567		97.0 (chain: 1, length: 132)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 132, Sequence coverage: 97.0%
- Total number of assignments
  - ¹H chemical shifts: 894
  - ¹³C chemical shifts: 545
  - ¹⁵N chemical shifts: 128
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	834	718	86.1
¹³C chemical shifts	624	545	87.3
¹⁵N chemical shifts	144	128	88.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	267	256	95.9
¹³C chemical shifts	264	254	96.2
¹⁵N chemical shifts	128	120	93.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	567	462	81.5
¹³C chemical shifts	360	291	80.8
¹⁵N chemical shifts	16	8	50.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	93	88	94.6
¹³C chemical shifts	93	86	92.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	46	7	15.2
¹³C chemical shifts	46	0	0.0

Keywords mutant, Spo0F, sporulation, surface recognition

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Found 1 Document (0.67 seconds)

BMRB: 15011

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. Spo0FY13A monomer, : 1 : 8 : 275 entities Detail

Interaction partners 1. Spo0FY13A monomer, : 6 interactors Detail

Experiments performed 8 experiments Detail

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Chemical shift validation 3 contents Detail