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BMRB: 16009

4KL5

NMR resonance assignment of DnaE intein from Nostoc punctiforme

Authors

Heinamaki, K., Djupsjobacka, J., Oemig, J.S., Iwai, H.

Assembly

NpuDnaE intein

Entity

1. NpuDnaE intein (polymer, Thiol state: all free), 139 monomers, 15914.82 Da Detail

GGALSYETEI LTVEYGLLPI GKIVEKRIEC TVYSVDNNGN IYTQPVAQWH DRGEQEVFEY CLEDGSLIRA TKDHKFMTVD GQMLPIDEIF ERELDLMRVD NLPNIKIATR KYLGKQNVYD IGVERDHNFA LKNGFIASN

Formula weight

15914.82 Da

Source organism

Nostoc punctiforme

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 94.5 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.5 % (1553 of 1643)	95.0 % (811 of 854)	94.1 % (601 of 639)	94.0 % (141 of 150)
Backbone	97.1 % (802 of 826)	98.2 % (280 of 285)	96.8 % (393 of 406)	95.6 % (129 of 135)
Sidechain	92.7 % (876 of 945)	93.3 % (531 of 569)	92.2 % (333 of 361)	80.0 % (12 of 15)
Aromatic	81.5 % (106 of 130)	90.8 % (59 of 65)	71.9 % (46 of 64)	100.0 % (1 of 1)
Methyl	99.4 % (161 of 162)	98.8 % (80 of 81)	100.0 % (81 of 81)

1. intein polypeptide

GGALSYETEI LTVEYGLLPI GKIVEKRIEC TVYSVDNNGN IYTQPVAQWH DRGEQEVFEY CLEDGSLIRA TKDHKFMTVD GQMLPIDEIF ERELDLMRVD NLPNIKIATR KYLGKQNVYD IGVERDHNFA LKNGFIASN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	intein_polypeptide	[U-100% 13C; U-100% 15N]		2 mM
2	sodium phosphate	natural abundance		10 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
3	intein_polypeptide	[U-20% 13C; U-100% 15N]		2 mM
4	sodium phosphate	natural abundance		10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.52 ppm, Outliers: 4 Detail

Release date

2009-01-14

Citation 1

NMR resonance assignment of DnaE intein from Nostoc punctiforme
Heinamaki, K., Oeemig, J.S., Djupsjobacka, J., Iwai, H.
Biomol. NMR Assign. (2009), 3, 41-43, PubMed 19636943 , DOI 10.1007/s12104-008-9137-1 , Abstract

Show more 1 citaion

Citation 2

Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme
Iwai, H., Zuger, S., Jin, J., Tam, P.
FEBS Lett. (2006), 580, 1853-1858, PubMed 16516207 , DOI 10.1016/j.febslet.2006.02.045 , Abstract

Hide non-primary 1 citaion

Entries sharing articles BMRB: 4 entries Detail

  BMRB: 26979 released on 2016-12-27
    Title Wild type fused Npu DnaE from Nostoc punctiforme with Phe +2 Extein
  BMRB: 26980 released on 2016-12-27
    Title Wild type fused Npu DnaE from Nostoc punctiforme with Gly +2 Extein
  BMRB: 26981 released on 2016-12-27
    Title Fused Npu DnaE GEP loop mutant from Nostoc punctiforme with Phe +2 Extein
  BMRB: 26984 released on 2016-12-27
    Title Fused Npu DnaE GEP loop mutant from Nostoc punctiforme with Phe +2 Extein

Related entities 1. NpuDnaE intein, : 1 : 1 : 14 entities Detail

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC