BMRB: 26980

Wild type fused Npu DnaE from Nostoc punctiforme with Gly +2 Extein

Authors

Sekar, G., Cowburn, D.

Assembly

Wild type fused Npu DnaE from Nostoc punctiforme with Gly +2 Extein

Entity

1. Wild type fused Npu DnaE from Nostoc punctiforme with Gly +2 Extein (polymer, Thiol state: all free), 144 monomers, 16526.55 Da Detail

AEYALSYETE ILTVEYGLLP IGKIVEKRIE CTVYSVDNNG NIYTQPVAQW HDRGEQEVFE YCLEDGSLIR ATKDHKFMTV DGQMLPIDEI FERELDLMRV DNLPNMIKIA TRKYLGKQNV YDIGVERDHN FALKNGFIAS ACGN

Formula weight

16526.55 Da

Source organism

Nostoc punctiforme

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 47.1 %, Completeness (bb): 82.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	47.1 % (801 of 1699)	25.3 % (223 of 882)	65.1 % (431 of 662)	94.8 % (147 of 155)
Backbone	82.6 % (707 of 856)	52.0 % (153 of 294)	98.8 % (417 of 422)	97.9 % (137 of 140)
Sidechain	23.2 % (227 of 977)	11.9 % (70 of 588)	39.3 % (147 of 374)	66.7 % (10 of 15)
Aromatic	10.1 % (14 of 138)	10.1 % (7 of 69)	8.8 % (6 of 68)	100.0 % (1 of 1)
Methyl	7.8 % (13 of 166)	3.6 % (3 of 83)	12.0 % (10 of 83)

1. Npu DnaE

AEYALSYETE ILTVEYGLLP IGKIVEKRIE CTVYSVDNNG NIYTQPVAQW HDRGEQEVFE YCLEDGSLIR ATKDHKFMTV DGQMLPIDEI FERELDLMRV DNLPNMIKIA TRKYLGKQNV YDIGVERDHN FALKNGFIAS ACGN

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	sodium phosphate	natural abundance		20 mM
2	TCEP	natural abundance		2 mM
3	Npu DnaE	[U-99% 13C; U-99% 15N]		400 mM

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.52 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Release date

2016-12-27

Citation 1

A promiscuous split intein with expanded protein engineering applications
Stevens, A.J., Sekar, G., Shah, N.H., Mostafavi, A., Cowburn, D., Muir, T.W.
Proc. Natl. Acad. Sci. U. S. A. (2017), 114, 8538-8543, PubMed 28739907 , DOI 10.1073/pnas.1701083114 , Abstract

Show more 1 citaion

Citation 2

NMR resonance assignment of DnaE intein from Nostoc punctiforme
Kimmo, H., Jesper, O., Janica, D., Hideo, I.
Biomol. NMR Assign. (2009), 3, 41-43, PubMed 19636943 , DOI 10.1007/s12104-008-9137-1 , Abstract

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Entries sharing articles BMRB: 4 entries Detail

Related entities 1. Wild type fused Npu DnaE from Nostoc punctiforme with Gly +2 Extein, : 14 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail