BMRB: 26981

Fused Npu DnaE GEP loop mutant from Nostoc punctiforme with Phe +2 Extein

Authors

Sekar, G., Cowburn, D.

Assembly

Fused Npu DnaE GEP loop mutant from Nostoc punctiforme with Phe +2 Extein

Entity

1. Fused Npu DnaE GEP loop mutant from Nostoc punctiforme with Phe +2 Extein (polymer, Thiol state: all free), 144 monomers, 16499.56 Da Detail

AEYALSYETE ILTVEYGLLP IGKIVEKRIE CTVYSVDNNG NIYTQPVAQW HDRGEQEVFE YCLEDGSLIR ATKDHKFMTV DGQMLPIDEI FERELDLMRV DNLPNMIKIA TRKYLGKQNV YDIGVGEPHN FALKNGFIAS ACFN

Formula weight

16499.56 Da

Source organism

Nostoc punctiforme

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 44.8 %, Completeness (bb): 82.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	44.8 % (765 of 1706)	21.4 % (189 of 885)	64.9 % (433 of 667)	92.9 % (143 of 154)
Backbone	82.2 % (702 of 854)	50.9 % (149 of 293)	99.1 % (418 of 422)	97.1 % (135 of 139)
Sidechain	19.8 % (195 of 986)	6.8 % (40 of 592)	38.8 % (147 of 379)	53.3 % (8 of 15)
Aromatic	1.4 % (2 of 148)	1.4 % (1 of 74)	0.0 % (0 of 73)	100.0 % (1 of 1)
Methyl	12.0 % (20 of 166)	4.8 % (4 of 83)	19.3 % (16 of 83)

1. Npu DnaE

AEYALSYETE ILTVEYGLLP IGKIVEKRIE CTVYSVDNNG NIYTQPVAQW HDRGEQEVFE YCLEDGSLIR ATKDHKFMTV DGQMLPIDEI FERELDLMRV DNLPNMIKIA TRKYLGKQNV YDIGVGEPHN FALKNGFIAS ACFN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	sodium phosphate	natural abundance		20 mM
2	TCEP	natural abundance		2 mM
3	Npu DnaE	[U-99% 13C; U-99% 15N]		400 mM

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.43 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Release date

2016-12-27

Citation 1

A promiscuous split intein with expanded protein engineering applications
Stevens, A.J., Sekar, G., Shah, N.H., Mostafavi, A., Cowburn, D., Muir, T.W.
Proc. Natl. Acad. Sci. U. S. A. (2017), 114, 8538-8543, PubMed 28739907 , DOI 10.1073/pnas.1701083114 , Abstract

Show more 1 citaion

Citation 2

NMR resonance assignment of DnaE intein from Nostoc punctiforme
Kimmo, H., Jesper, O., Janica, D., Hideo, I.
Biomol. NMR Assign. (2009), 3, 41-43, PubMed 19636943 , DOI 10.1007/s12104-008-9137-1 , Abstract

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Entries sharing articles BMRB: 4 entries Detail

Related entities 1. Fused Npu DnaE GEP loop mutant from Nostoc punctiforme with Phe +2 Extein, : 14 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail