Structure of the three-Cys2His2 domain of mouse testis zinc finger protein
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 92.1 % (291 of 316) | 94.6 % (159 of 168) | 86.9 % (106 of 122) | 100.0 % (26 of 26) |
Backbone | 98.1 % (155 of 158) | 100.0 % (53 of 53) | 96.2 % (77 of 80) | 100.0 % (25 of 25) |
Sidechain | 88.0 % (162 of 184) | 92.2 % (106 of 115) | 80.9 % (55 of 68) | 100.0 % (1 of 1) |
Aromatic | 32.1 % (9 of 28) | 57.1 % (8 of 14) | 7.1 % (1 of 14) | |
Methyl | 100.0 % (14 of 14) | 100.0 % (7 of 7) | 100.0 % (7 of 7) |
1. mTZD finger2
EKPFSCSLCP QRSRDFSAMT KHLRTHGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mTZD finger2 | 0.0 ~ 0.0 mM | ||
2 | acetic acid | [U-99% 2H] | 50 mM | |
3 | sodium chloride | natural abundance | 20 mM |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints | combined_16797_2kvh.nef |
Input source #2: Coordindates | 2kvh.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | True (see coordinates for details) |
Whether the assembly has a other bond | True (see coodinates for details) |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
Ptnr_site_1 | Ptnr_site_2 | Redox_state_prediction_1 | Redox_state_prediction_2 | Distance (Å) |
---|---|---|---|---|
A:34:CYS:SG | A:37:CYS:SG | reduced, CA 60.066, CB 29.527 ppm | reduced, CA 58.14, CB 29.46 ppm | 2.846 |
Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
Ptnr_site_1 | Ptnr_site_2 | Redox_state_prediction_1 | Redox_state_prediction_2 | Distance (Å) |
---|---|---|---|---|
1:22:HIS:NE2 | 2:1:ZN:ZN | unknown | unknown | n/a |
1:26:HIS:NE2 | 2:1:ZN:ZN | unknown | unknown | n/a |
1:9:CYS:SG | 2:1:ZN:ZN | unknown | unknown | n/a |
1:6:CYS:SG | 2:1:ZN:ZN | unknown | unknown | n/a |
Non-standard residues
Chain_ID | Seq_ID | Comp_ID | Chem_comp_name | Experimental evidences |
---|---|---|---|---|
B | 1 | ZN | ZINC ION | Distance restraints |
Sequence alignments
-30-------40--------50----- EKPFSCSLCPQRSRDFSAMTKHLRTHG ||||||||||||||||||||||||||| EKPFSCSLCPQRSRDFSAMTKHLRTHG --------10--------20-------
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 27 | 0 | 0 | 100.0 |
Content subtype: combined_16797_2kvh.nef
Assigned chemical shifts
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 168 | 158 | 94.0 |
13C chemical shifts | 122 | 106 | 86.9 |
15N chemical shifts | 29 | 26 | 89.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 53 | 52 | 98.1 |
13C chemical shifts | 54 | 51 | 94.4 |
15N chemical shifts | 25 | 25 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 115 | 106 | 92.2 |
13C chemical shifts | 68 | 55 | 80.9 |
15N chemical shifts | 4 | 1 | 25.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 8 | 8 | 100.0 |
13C chemical shifts | 8 | 8 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 14 | 8 | 57.1 |
13C chemical shifts | 14 | 1 | 7.1 |
Covalent bonds
Distance restraints
-30-------40--------50----- EKPFSCSLCPQRSRDFSAMTKHLRTHG ||||||||||||||||||||||||||| EKPFSCSLCPQRSRDFSAMTKHLRTHG
Dihedral angle restraints
-30-------40--------50----- EKPFSCSLCPQRSRDFSAMTKHLRTHG ||||||||| ||| ||||||||| EKPFSCSLC..RSR.FSAMTKHLR -30-------40--------50--