Backbone dynamics of Tryptophan repressor L75F mutant protein in holo-form
HHHHHHAQQS PYSAAMAEQR HQEWLRFVDL LKNAYQNDLH LPLLNLMLTP DEREALGTRV RIVEELLRGE MSQRELKNEF GAGIATITRG SNSLKAAPVE LRQWLEEVLL KSD
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 32.8 % (441 of 1344) | 18.9 % (133 of 703) | 40.2 % (208 of 518) | 81.3 % (100 of 123) |
Backbone | 59.7 % (400 of 670) | 46.7 % (106 of 227) | 58.4 % (195 of 334) | 90.8 % (99 of 109) |
Sidechain | 16.5 % (129 of 782) | 5.5 % (26 of 476) | 35.3 % (103 of 292) | 0.0 % (0 of 14) |
Aromatic | 0.0 % (0 of 92) | 0.0 % (0 of 46) | 0.0 % (0 of 44) | 0.0 % (0 of 2) |
Methyl | 11.4 % (15 of 132) | 4.5 % (3 of 66) | 18.2 % (12 of 66) |
1. TrpR L75F
HHHHHHAQQS PYSAAMAEQR HQEWLRFVDL LKNAYQNDLH LPLLNLMLTP DEREALGTRV RIVEELLRGE MSQRELKNEF GAGIATITRG SNSLKAAPVE LRQWLEEVLL KSDSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TrpR | [U-100% 13C; U-100% 15N] | 1 mM | |
2 | L-Tryptophan | natural abundance | 5 mM | |
3 | D2O | natural abundance | 5 % | |
4 | H2O | natural abundance | 95 % | |
5 | PMSF | natural abundance | 0.1 mM | |
6 | sodium azide | natural abundance | 0.01 % | |
7 | sodium chloride | natural abundance | 500 mM | |
8 | sodium phosphate | natural abundance | 50 mM | |
9 | EDTA | natural abundance | 1 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | TrpR | [U-100% 13C; U-100% 15N] | 1 mM | |
11 | L-Tryptophan | natural abundance | 5 mM | |
12 | D2O | natural abundance | 5 % | |
13 | H2O | natural abundance | 95 % | |
14 | PMSF | natural abundance | 0.1 mM | |
15 | sodium azide | natural abundance | 0.01 % | |
16 | sodium chloride | natural abundance | 500 mM | |
17 | sodium phosphate | natural abundance | 50 mM | |
18 | EDTA | natural abundance | 1 mM |