BMRBj - BMREntryMaster

Found 1 Document (1.059 seconds)

BMRB: 2071

Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR

Authors

Meiering, E.M., Bycroft, M., Fersht, A.R.

Assembly

barnase

Source organism

Bacillus amyloliquefaciens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 36.7 %, Completeness: 30.6 %, Completeness (bb): 58.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	30.6 % (79 of 258)	30.6 % (79 of 258)
Backbone	58.1 % (50 of 86)	58.1 % (50 of 86)
Sidechain	16.9 % (29 of 172)	16.9 % (29 of 172)
Aromatic	18.5 % (5 of 27)	18.5 % (5 of 27)
Methyl	16.7 % (3 of 18)	16.7 % (3 of 18)

1. barnase

XXXXXXFXXV AXXLXXXXXX XXNXXXKXXX QXXXXXXXXG XXXXXXXXKX IGGDXFXNXX XXXXXXXXRT WREADINXXX GXXNSDRXLX XSXXXIXKTT XHYQTFXXK

Show sample condition

Sample

Temperature 300 K, pH 4.5

Release date

1995-07-30

Citation

Characterization of phosphate binding in the active site of barnase by site-directed mutagenesis and NMR
Meiering, E.M., Bycroft, M., Fersht, A.R.
Biochemistry (1991), 30, 11348-11356, PubMed 1958671 , Abstract

Entries sharing articles BMRB: 5 entries Detail

  BMRB: 2066 released on 1995-07-30
    Title Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
  BMRB: 2067 released on 1995-07-30
    Title Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
  BMRB: 2068 released on 1995-07-30
    Title Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
  BMRB: 2069 released on 1995-07-30
    Title Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR
  BMRB: 2070 released on 1995-07-30
    Title Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr2071_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (1.059 seconds)

BMRB: 2071

Sample

Entries sharing articles BMRB: 5 entries Detail

Related entities 1. barnase, : 58 entities Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail