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BMRB: 25248

5LG7

1H, 13C, and 15N Chemical Shift Assignments for the W972R mutant of Arkadia (RNF111) E3 RING domain

Authors

Chasapis, C.T., Birkou, M., Herrmann, T., Episkopou, V., Bentrop, D., Spyroulias, G.A.

Assembly

RING Finger monomer

Entity

1. W972R (polymer, Thiol state: all free), 69 monomers, 7920.935 Da Detail

MKQDGEEGTE EDTEEKCTIC LSILEEGEDV RRLPCMHLFH QVCVDQRLIT NKKCPICRVD IEAQLPSES

2. ZINC ION (non-polymer), 65.409 Da

Total weight

7986.344 Da

Max. entity weight

7920.935 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

molecular mechanics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 54.6 %, Completeness (bb): 63.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	54.6 % (432 of 791)	48.4 % (202 of 417)	54.8 % (166 of 303)	90.1 % (64 of 71)
Backbone	63.7 % (260 of 408)	49.3 % (68 of 138)	62.7 % (128 of 204)	97.0 % (64 of 66)
Sidechain	51.4 % (231 of 449)	48.0 % (134 of 279)	58.8 % (97 of 165)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 18)	0.0 % (0 of 9)	0.0 % (0 of 9)
Methyl	25.7 % (18 of 70)	5.7 % (2 of 35)	45.7 % (16 of 35)

1. W972R

MKQDGEEGTE EDTEEKCTIC LSILEEGEDV RRLPCMHLFH QVCVDQRLIT NKKCPICRVD IEAQLPSES

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	W972R	[U-99% 15N]	0.6 mM
2	phosphate buffer	natural abundance	50 mM
3	H20	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	W972R	[U-98% 13C; U-98% 15N]	0.6 mM
6	phosphate buffer	natural abundance	50 mM
7	H20	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.04 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 31 models in PDB: 5LG7, Strand ID: A Detail

Release date

2014-09-24

Citation

A residue-specific insight into the Arkadia E3 ubiquitin ligase activity and conformational plasticity
Birkou, M., Chasapis, C.T., Herrmann, T., Episkopou, V., Bentrop, D., Spyroulias, G.A.
J. Mol. Biol. (2017), PubMed 28647409 , DOI 10.1016/j.jmb.2017.06.012 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 25351 released on 2014-11-18
    Title 1H, 13C and 15N Chemical Shift Assignments of the H962C mutant from Arkadia (RNF111) E3 RING domain
  BMRB: 25249 released on 2014-09-24
    Title 1H, 13C, and 15N Chemical Shift Assignments for the W972A mutant of Arkadia (RNF111) E3 RING domain

Related entities 1. W972R, : 1 : 9 : 123 entities Detail

Interaction partners 1. W972R, : 38 interactors Detail

More details for 38 interactors identified by 11 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC