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BMRB: 25351

2KIZ

1H, 13C and 15N Chemical Shift Assignments of the H962C mutant from Arkadia (RNF111) E3 RING domain

Authors

Marousis, K.D., Tsapardoni, S., Birkou, M., Chasapis, C.T., Bentrop, D., Spyroulias, G.A.

Assembly

RING finger monomer

Entity

1. RING finger monomer, entity (polymer, Thiol state: all free), 69 monomers, 7916.962 Da Detail

MKQDGEEGTE EDTEEKCTIC LSILEEGEDV RRLPCMCLFH QVCVDQWLIT NKKCPICRVD IEAQLPSES

2. RING finger monomer, entity ZN (non-polymer), 65.409 × 2 Da

Total weight

8047.78 Da

Max. entity weight

7916.962 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.2 %, Completeness (bb): 99.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.2 % (730 of 792)	89.9 % (374 of 416)	95.7 % (291 of 304)	90.3 % (65 of 72)
Backbone	99.5 % (406 of 408)	99.3 % (137 of 138)	100.0 % (204 of 204)	98.5 % (65 of 66)
Sidechain	86.7 % (390 of 450)	85.3 % (237 of 278)	92.2 % (153 of 166)	0.0 % (0 of 6)
Aromatic	65.4 % (17 of 26)	69.2 % (9 of 13)	66.7 % (8 of 12)	0.0 % (0 of 1)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. entity

MKQDGEEGTE EDTEEKCTIC LSILEEGEDV RRLPCMCLFH QVCVDQWLIT NKKCPICRVD IEAQLPSES

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	H962C	[U-99% 15N]	0.40 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
4	H962C	[U-98% 13C; U-98% 15N]	0.30 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 2 Detail

Release date

2014-11-18

Citation

A residue-specific insight into the Arkadia E3 ubiquitin ligase activity and conformational plasticity
Birkou, M., Chasapis, C.T., Marousis, K.D., Loutsidou, A.K., Bentrop, D., Lelli, M., Herrmann, T., Carthy, J.M., Episkopou, V., Spyroulias, G.A.
J. Mol. Biol. (2017), 429, 2373-2386, PubMed 28647409 , DOI 10.1016/j.jmb.2017.06.012 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 25248 released on 2014-09-24
    Title 1H, 13C, and 15N Chemical Shift Assignments for the W972R mutant of Arkadia (RNF111) E3 RING domain
  BMRB: 25249 released on 2014-09-24
    Title 1H, 13C, and 15N Chemical Shift Assignments for the W972A mutant of Arkadia (RNF111) E3 RING domain

Related entities 1. RING finger monomer, entity, : 1 : 9 : 115 entities Detail

Interaction partners 1. RING finger monomer, entity, : 38 interactors Detail

More details for 38 interactors identified by 11 citations