1H, 13C, and 15N Chemical Shift Assignments for the W972A mutant of Arkadia (RNF111) E3 RING domain
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 55.6 % (435 of 783) | 49.6 % (204 of 411) | 55.8 % (168 of 301) | 88.7 % (63 of 71) |
Backbone | 61.8 % (252 of 408) | 48.6 % (67 of 138) | 60.8 % (124 of 204) | 92.4 % (61 of 66) |
Sidechain | 54.4 % (240 of 441) | 50.2 % (137 of 273) | 62.0 % (101 of 163) | 40.0 % (2 of 5) |
Aromatic | 0.0 % (0 of 18) | 0.0 % (0 of 9) | 0.0 % (0 of 9) | |
Methyl | 29.2 % (21 of 72) | 0.0 % (0 of 36) | 58.3 % (21 of 36) |
1. W972A
MKQDGEEGTE EDTEEKCTIC LSILEEGEDV RRLPCMHLFH QVCVDQALIT NKKCPICRVD IEAQLPSESSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | W972A | [U-99% 15N] | 0.6 mM | |
2 | phosphate buffer | natural abundance | 50 mM | |
3 | H20 | natural abundance | 90 % | |
4 | D20 | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | W972A | [U-98% 13C; U-98% 15N] | 0.6 mM | |
6 | phosphate buffer | natural abundance | 50 mM | |
7 | H20 | natural abundance | 90 % | |
8 | D20 | natural abundance | 10 % |