BMRBj - BMREntryMaster

Found 1 Document (2.762 seconds)

BMRB: 28063

6LTH

Backbone 1H, 13C, and 15N Chemical shift Assignments for SWIRM domain of BAF155 and RPT1 of hSNF5

Authors

Han, J., Kim, I., Suh, J., Lee, W.

Assembly

SWIRM with RPT1 heterodimer

Entity

1. SWIRM (polymer, Thiol state: all free), 98 monomers, 11598.16 Da Detail

IIIPSYASWF DYNCIHVIER RALPEFFNGK NKSKTPEIYL AYRNFMIDTY RLNPQEYLTS TACRRNLTGD VCAVMRVHAF LEQWGLVNYQ VDPESRPM

2. RPT1 (polymer, Thiol state: all free), 88 monomers, 10012.18 Da Detail

HDPAVIHENA SQPEVLVPIR LDMEIDGQKL RDAFTWNMNE KLMTPEMFSE ILCDDLDLNP LTFVPAIASA IRQQIESYPT DSILEDQS

Total weight

21610.34 Da

Max. entity weight

11598.16 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 51.1 %, Completeness: 15.3 %, Completeness (bb): 30.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	15.3 % (342 of 2232)	7.9 % (91 of 1156)	19.0 % (165 of 870)	41.7 % (86 of 206)
Backbone	30.6 % (334 of 1090)	24.0 % (87 of 363)	29.1 % (161 of 554)	49.7 % (86 of 173)
Sidechain	5.3 % (70 of 1324)	0.5 % (4 of 793)	13.3 % (66 of 498)	0.0 % (0 of 33)
Aromatic	0.0 % (0 of 196)	0.0 % (0 of 98)	0.0 % (0 of 95)	0.0 % (0 of 3)
Methyl	3.9 % (8 of 206)	0.0 % (0 of 103)	7.8 % (8 of 103)

1. SWIRM

IIIPSYASWF DYNCIHVIER RALPEFFNGK NKSKTPEIYL AYRNFMIDTY RLNPQEYLTS TACRRNLTGD VCAVMRVHAF LEQWGLVNYQ VDPESRPM

2. RPT1

HDPAVIHENA SQPEVLVPIR LDMEIDGQKL RDAFTWNMNE KLMTPEMFSE ILCDDLDLNP LTFVPAIASA IRQQIESYPT DSILEDQS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	SWIRM	[U-99% 13C; U-99% 15N]	0.5 mM
2	RPT1	natural abundance	0.5 mM
3	HEPES	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	DTT	natural abundance	2 mM

Release date

2020-01-21

Citation

A Coil-to-Helix Transition Serves as a Binding Motif for hSNF5 and BAF155 Interaction
Han, J., Kim, I., Park, J.H., Yun, J.H., Joo, K., Kim, T., Park, G.Y., Ryu, K.S., Ko, Y.J., Mizutani, K., Park, S.Y., Seong, R.H., Lee, J., Suh, J.Y., Lee, W.
Int. J. Mol. Sci. (2020), 21, 2452-2452, PubMed 32244797 , DOI 10.3390/ijms21072452 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 28062 released on 2020-01-21
    Title Backbone 1H, 13C, and 15N Chemical Shift Assignments for RPT1 of hSNF5 and SWIRM of BAF155
  BMRB: 28064 released on 2020-01-21
    Title Solution structure of hSNF5 RPT1 domain

Related entities 1. SWIRM, : 3 : 50 entities Detail

Related entities 2. RPT1, : 1 : 8 : 4 : 26 entities Detail

Interaction partners 2. RPT1, : 17 interactors Detail

More details for 17 interactors identified by 10 citations

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr28063_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr28063_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	322		95.9 (chain: 1, length: 98)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 98, Sequence coverage: 95.9%
- Total number of assignments
  - ¹³C chemical shifts: 152
  - ¹H chemical shifts: 85
  - ¹⁵N chemical shifts: 85
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	469	152	32.4
¹H chemical shifts	623	85	13.6
¹⁵N chemical shifts	112	85	75.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	196	93	47.4
¹H chemical shifts	193	85	44.0
¹⁵N chemical shifts	92	85	92.4

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Found 1 Document (2.762 seconds)

BMRB: 28063

Sample

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. SWIRM, : 3 : 50 entities Detail

Related entities 2. RPT1, : 1 : 8 : 4 : 26 entities Detail

Interaction partners 2. RPT1, : 17 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	273	59	21.6
¹H chemical shifts	430	0	0.0
¹⁵N chemical shifts	20	0	0.0