NMR assignment and structure of a peptide derived from the fusion peptide of HIV-1 gp41 in the presence of dodecylphosphocholine micelles
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 69.0 % (254 of 368) | 98.1 % (207 of 211) | 29.9 % (47 of 157) |
Backbone | 64.1 % (109 of 170) | 98.6 % (72 of 73) | 38.1 % (37 of 97) |
Sidechain | 72.7 % (165 of 227) | 97.8 % (135 of 138) | 33.7 % (30 of 89) |
Aromatic | 50.0 % (21 of 42) | 95.5 % (21 of 22) | 0.0 % (0 of 20) |
Methyl | 70.0 % (28 of 40) | 100.0 % (20 of 20) | 40.0 % (8 of 20) |
1. entity 1
GIGALFLGFL GAAGSKKXKN EQELLELDKW ASLWNSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.6 mM wtFP-tag, 20 mM [U-98% 2H] DPC, 2.0 mM HEPES, 90 % H2O, 10 % [U-99% 2H] D2O, 0.1 mM DSS.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | D2O | [U-99% 2H] | 10 % | |
2 | DPC | [U-98% 2H] | 20 mM | |
3 | DSS | natural abundance | 0.1 mM | |
4 | H2O | natural abundance | 90 % | |
5 | HEPES | natural abundance | 2.0 mM | |
6 | wtFP-tag | natural abundance | 0.6 (±0.1) mM |