NMR assignment and structure of a peptide derived from the fusion peptide of HIV-1 gp41 in the presence of hexafluoroisopropanol
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.8 % (329 of 407) | 96.7 % (204 of 211) | 57.3 % (90 of 157) | 89.7 % (35 of 39) |
Backbone | 78.4 % (160 of 204) | 98.6 % (72 of 73) | 59.8 % (58 of 97) | 88.2 % (30 of 34) |
Sidechain | 84.5 % (196 of 232) | 95.7 % (132 of 138) | 66.3 % (59 of 89) | 100.0 % (5 of 5) |
Aromatic | 45.5 % (20 of 44) | 81.8 % (18 of 22) | 0.0 % (0 of 20) | 100.0 % (2 of 2) |
Methyl | 97.5 % (39 of 40) | 100.0 % (20 of 20) | 95.0 % (19 of 20) |
1. entity 1
GIGALFLGFL GAAGSKKXKN EQELLELDKW ASLWNSolvent system hexafluoroisopropanol/water, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.6 mM wtFP-tag, 25 % [U-98% 2H] HFIP, 2.0 mM HEPES, 67.5 % H2O, 7.5 % [U-99% 2H] D2O, 0.1 mM DSS, hexafluoroisopropanol/water.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | D2O | [U-99% 2H] | 7.5 % | |
2 | DSS | natural abundance | 0.1 mM | |
3 | H2O | natural abundance | 67.5 % | |
4 | HEPES | natural abundance | 2.0 mM | |
5 | HFIP | [U-98% 2H] | 25 % | |
6 | wtFP-tag | natural abundance | 0.6 (±0.1) mM |