BMRBj - BMREntryMaster

Found 1 Document (1.055 seconds)

BMRB: 34710

7YYI

Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) TMD

Authors

Silber, M., Muhle-Goll, C.

Assembly

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155)

Entity

1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155) (polymer, Thiol state: not present), 29 monomers, 3544.366 Da Detail

KKKLGFFLVT FGFIWGMMLL HFTIQQRKK

Formula weight

3544.366 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 72.3 %, Completeness (bb): 79.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.3 % (295 of 408)	76.6 % (164 of 214)	61.5 % (99 of 161)	97.0 % (32 of 33)
Backbone	79.9 % (139 of 174)	90.2 % (55 of 61)	65.5 % (55 of 84)	100.0 % (29 of 29)
Sidechain	70.0 % (182 of 260)	71.2 % (109 of 153)	68.0 % (70 of 103)	75.0 % (3 of 4)
Aromatic	45.5 % (30 of 66)	57.6 % (19 of 33)	31.3 % (10 of 32)	100.0 % (1 of 1)
Methyl	87.5 % (28 of 32)	87.5 % (14 of 16)	87.5 % (14 of 16)

1. entity 1

KKKLGFFLVT FGFIWGMMLL HFTIQQRKK

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 300 K, pH 7.0, Details 0.500 mM GNTV_WT, trifluoroethanol/water

#	Name	Isotope labeling	Type	Concentration
1	GNTV_WT	natural abundance		0.500 mM

Release date

2022-03-22

Citation

Helical stability of the GnTV transmembrane domain impacts on SPPL3 dependent cleavage
Papadopoulou, A.A., Stelzer, W., Silber, M., Schlosser, C., Spitz, C., Haug-Kroper, M., Straub, T., Muller, S.A., Lichtenthaler, S.F., Muhle-Goll, C., Langosch, D., Fluhrer, R.
Sci. Rep. (2022), 12, 20987-20987, PubMed 36470941 , DOI 10.1038/s41598-022-24772-8 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 34711 released on 2022-03-16
    Title Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) G26P mutant TMD
  BMRB: 34712 released on 2022-03-16
    Title Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) G22L mutant TMD
  BMRB: 34713 released on 2022-03-16
    Title Solution NMR structure of N-acetylglucosaminyltransferase V (GnTV) G22L and G26L double mutant TMD

Related entities 1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155), : 1 : 3 : 13 entities Detail

Interaction partners 1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155), : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34710_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34710_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	319		96.6 (chain: 1, length: 29)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 29, Sequence coverage: 96.6%
- Total number of assignments
  - ¹H chemical shifts: 195
  - ¹³C chemical shifts: 93
  - ¹⁵N chemical shifts: 31
- Completeness of assignments
  - Entity_assemble_ID: 1
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
15	TRP	CE2	120.084

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	214	167	78.0
¹³C chemical shifts	161	92	57.1
¹⁵N chemical shifts	33	31	93.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	61	58	95.1
¹³C chemical shifts	58	28	48.3
¹⁵N chemical shifts	29	28	96.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	153	109	71.2
¹³C chemical shifts	103	64	62.1
¹⁵N chemical shifts	4	3	75.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	18	14	77.8
¹³C chemical shifts	18	14	77.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	33	18	54.5
¹³C chemical shifts	32	8	25.0
¹⁵N chemical shifts	1	1	100.0

Keywords Glycosyltransferase, GnTV, Golgi, MEMBRANE PROTEIN, SPPL3, TMD, intramembrane

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.055 seconds)

BMRB: 34710

Sample

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155), : 1 : 3 : 13 entities Detail

Interaction partners 1. Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (E.C.2.4.1.155), : 2 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail