BMRBj - BMREntryMaster

Found 1 Document (3.19 seconds)

BMRB: 50168

7ZHS

J-domain region of DnaJA2

Authors

Rosenzweig, R., Abayev-Avraham, M.

Assembly

J-domain region of DnaJA2

Entity

1. J-domain region of DnaJA2 (polymer, Thiol state: all free), 77 monomers, 8777.710 Da Detail

MANVADTKLY DILGVPPGAS ENELKKAYRK LAKEYHPDKN PNAGDKFKEI SFAYEVLSNP EKRELYDRYG EQGLREG

Formula weight

8777.71 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 37.1 %, Completeness (bb): 75.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	37.1 % (341 of 919)	14.0 % (68 of 485)	58.2 % (205 of 352)	82.9 % (68 of 82)
Backbone	75.2 % (340 of 452)	43.9 % (68 of 155)	90.7 % (204 of 225)	94.4 % (68 of 72)
Sidechain	11.9 % (64 of 538)	0.0 % (0 of 330)	32.3 % (64 of 198)	0.0 % (0 of 10)
Aromatic	0.0 % (0 of 72)	0.0 % (0 of 36)	0.0 % (0 of 36)
Methyl	10.9 % (7 of 64)	0.0 % (0 of 32)	21.9 % (7 of 32)

1. J-domain of DnaJA2

MANVADTKLY DILGVPPGAS ENELKKAYRK LAKEYHPDKN PNAGDKFKEI SFAYEVLSNP EKRELYDRYG EQGLREG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	50 mM
2	KCl	natural abundance	50 mM
3	sodium azide	natural abundance	0.03 %
4	D2O	[U-2H]	10 %

Release date

2020-01-26

Citation

HSP40 proteins use class-specific regulation to drive HSP70 functional diversity
Rosenzweig, R., Abayev-Avraham, M., Faust, O., Maurer, M., Wentink, A., Bukau, B., Nillegoda, N.B., London, N.
Nature (2020), 587, 489-494, PubMed 33177718 , DOI 10.1038/s41586-020-2906-4 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 50167 released on 2020-01-26
    Title DnaJB1 J-domain+G/F rich region
  BMRB: 50169 released on 2020-01-26
    Title DnaJB1 J-domain

Related entities 1. J-domain region of DnaJA2, : 1 : 7 : 242 entities Detail

Interaction partners 1. J-domain region of DnaJA2, : 67 interactors Detail

More details for 67 interactors identified by 36 citations

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr50168_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr50168_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1_A2JD	OK	342		96.1 (chain: 1, length: 77)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1_A2JD
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 77, Sequence coverage: 96.1%
- Total number of assignments
  - ¹H chemical shifts: 69
  - ¹³C chemical shifts: 204
  - ¹⁵N chemical shifts: 69
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	155	69	44.5
¹³C chemical shifts	154	141	91.6
¹⁵N chemical shifts	72	69	95.8

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Found 1 Document (3.19 seconds)

BMRB: 50168

Sample

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. J-domain region of DnaJA2, : 1 : 7 : 242 entities Detail

Interaction partners 1. J-domain region of DnaJA2, : 67 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	485	69	14.2
¹³C chemical shifts	352	204	58.0
¹⁵N chemical shifts	82	69	84.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	330	0	0.0
¹³C chemical shifts	198	63	31.8
¹⁵N chemical shifts	10	0	0.0