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BMRB: 50355

7PMM

1H, 13C, 15N backbone and ILMVA methyl group assignment of the N-terminal RecA domain of the DEAD-box RNA helicase DbpA from E.coli

Authors

Assembly

N-terminal RecA domain of DbpA

Entity

1. N-terminal RecA domain of DbpA (polymer, Thiol state: all free), 214 monomers, 22927.22 Da Detail

MTAFSTLNVL PPAQLTNLNE LGYLTMTPVQ AAALPAILAG KDVRVQAKTG SGKTAAFGLG LLQQIDASLF QTQALVLCPT RELADQVAGE LRRLARFLPN TKILTLCGGQ PFGMQRDSLQ HAPHIIVATP GRLLDHLQKG TVSLDALNTL VMDEADRMLD MGFSDAIDDV IRFAPASRQT LLFSATWPEA IAAISGRVQR DPLAIEIDST DALP

Formula weight

22927.22 Da

Source organism

Escherichia coli

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.6 %, Completeness: 67.5 %, Completeness (bb): 89.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	67.5 % (1632 of 2416)	61.3 % (757 of 1235)	71.7 % (680 of 948)	83.7 % (195 of 233)
Backbone	89.3 % (1124 of 1258)	88.1 % (378 of 429)	87.9 % (552 of 628)	96.5 % (194 of 201)
Sidechain	50.4 % (684 of 1358)	47.0 % (379 of 806)	58.5 % (304 of 520)	3.1 % (1 of 32)
Aromatic	1.8 % (2 of 112)	3.6 % (2 of 56)	0.0 % (0 of 55)	0.0 % (0 of 1)
Methyl	79.7 % (244 of 306)	80.4 % (123 of 153)	79.1 % (121 of 153)

1. entity 1

MTAFSTLNVL PPAQLTNLNE LGYLTMTPVQ AAALPAILAG KDVRVQAKTG SGKTAAFGLG LLQQIDASLF QTQALVLCPT RELADQVAGE LRRLARFLPN TKILTLCGGQ PFGMQRDSLQ HAPHIIVATP GRLLDHLQKG TVSLDALNTL VMDEADRMLD MGFSDAIDDV IRFAPASRQT LLFSATWPEA IAAISGRVQR DPLAIEIDST DALP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
5	N-terminal RecA domain	[U-15N; U-2H; 95% 1H13CD-Ile,Leu; 95% 1H13CG-Val, 1H13CB-Ala, 1H13CE-Met]		300 uM
6	sodium chloride	natural abundance		125 mM
7	HEPES	natural abundance		25 mM
8	DTT	natural abundance		1 mM

Release date

2020-06-24

Citation

Assignment of the Ile, Leu, Val, Met and Ala methyl group resonances of the DEAD-box RNA helicase DbpA from E. coli
Wurm, J.
Biomol. NMR Assign. (2021), 15, 121-128, PubMed 33277687 , DOI 10.1007/s12104-020-09994-z , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 50356 released on 2020-06-24
    Title 1H, 13C, 15N backbone and ILMVAT methyl group assignment of the C-terminal RecA and RBD domains of the DEAD-box RNA helicase DbpA from E.coli
  BMRB: 50357 released on 2020-06-24
    Title 1H, 15N backbone and ILMVA methyl group assignment of the DEAD-box RNA helicase DbpA from E.coli

Related entities 1. N-terminal RecA domain of DbpA, : 1 : 4 : 295 entities Detail

Interaction partners 1. N-terminal RecA domain of DbpA, : 3 interactors Detail

More details for 3 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

2 3D HNCACB

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

3 3D HNCO

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

4 3D HN(CA)CO

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

5 3D C(CO)NH

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

6 3D H(CCO)NH

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

7 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

8 3D CBCA(CO)NH

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

9 3D 1H-13C-1H NOESY methyl groups

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
5	N-terminal RecA domain	[U-15N; U-2H; 95% 1H13CD-Ile,Leu; 95% 1H13CG-Val, 1H13CB-Ala, 1H13CE-Met]		300 uM
6	sodium chloride	natural abundance		125 mM
7	HEPES	natural abundance		25 mM
8	DTT	natural abundance		1 mM

10 3D 13C-13C-1H NOESY methyl groups

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
5	N-terminal RecA domain	[U-15N; U-2H; 95% 1H13CD-Ile,Leu; 95% 1H13CG-Val, 1H13CB-Ala, 1H13CE-Met]		300 uM
6	sodium chloride	natural abundance		125 mM
7	HEPES	natural abundance		25 mM
8	DTT	natural abundance		1 mM

11 3D HBHA(CO)NH

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

12 3D HCCH-TOCSY

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

13 3D HCCH-COSY

Instrument

Bruker AVANCE NEO - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Type	Concentration
1	N-terminal RecA domain	[U-13C; U-15N]		400 uM
2	sodium chloride	natural abundance		125 mM
3	HEPES	natural abundance		25 mM
4	DTT	natural abundance		1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr50355_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr50355_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1854		88.8 (chain: 1, length: 214)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 214, Sequence coverage: 88.8%
- Total number of assignments
  - ¹H chemical shifts: 994
  - ¹³C chemical shifts: 671
  - ¹⁵N chemical shifts: 189
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1