BMRBj - BMREntryMaster

Found 1 Document (0.687 seconds)

BMRB: 50357

7PMQ

1H, 15N backbone and ILMVA methyl group assignment of the DEAD-box RNA helicase DbpA from E.coli

Authors

Wurm, J.

Assembly

full length DbpA

Entity

1. full length DbpA (polymer, Thiol state: all free), 457 monomers, 49187.18 Da Detail

MTAFSTLNVL PPAQLTNLNE LGYLTMTPVQ AAALPAILAG KDVRVQAKTG SGKTAAFGLG LLQQIDASLF QTQALVLCPT RELADQVAGE LRRLARFLPN TKILTLCGGQ PFGMQRDSLQ HAPHIIVATP GRLLDHLQKG TVSLDALNTL VMDEADRMLD MGFSDAIDDV IRFAPASRQT LLFSATWPEA IAAISGRVQR DPLAIEIDST DALPPIEQQF YETSSKGKIP LLQRLLSLHQ PSSCVVFCNT KKDCQAVCDA LNEVGQSALS LHGDLEQRDR DQTLVRFANG SARVLVATDV AARGLDIKSL ELVVNFELAW DPEVHVHRIG RTARAGNSGL AISFCAPEEA QRANIISDML QIKLNWQTPP ANSSIATLEA EMATLCIDGG KKAKMRPGDV LGALTGDIGL DGADIGKIAV HPAHVYVAVR QAVAHKAWKQ LQGGKIKGKT CRVRLLK

Formula weight

49187.18 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.4 %, Completeness: 33.7 %, Completeness (bb): 41.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.7 % (1743 of 5179)	32.1 % (853 of 2660)	23.3 % (469 of 2013)	83.2 % (421 of 506)
Backbone	41.6 % (1123 of 2698)	51.1 % (473 of 925)	17.3 % (232 of 1338)	96.1 % (418 of 435)
Sidechain	25.0 % (725 of 2905)	21.7 % (377 of 1735)	31.4 % (345 of 1099)	4.2 % (3 of 71)
Aromatic	5.8 % (14 of 242)	5.8 % (7 of 121)	3.4 % (4 of 117)	75.0 % (3 of 4)
Methyl	78.8 % (487 of 618)	78.6 % (243 of 309)	79.0 % (244 of 309)

1. entity 1

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.3

#	Name	Isotope labeling	Concentration
1	sodium chloride	natural abundance	125 mM
2	HEPES	natural abundance	25 mM
3	DTT	natural abundance	1 mM
4	DbpA full length	[U-15N; U-2H; 95% 1H13CD-Ile,Leu; 95% 1H13CG-Val, 1H13CB-Ala, 1H13CE-Met]	250 uM

Release date

2020-06-24

Citation

Assignment of the Ile, Leu, Val, Met and Ala methyl group resonances of the DEAD-box RNA helicase DbpA from E. coli
Wurm, J.
Biomol. NMR Assign. (2021), 15, 121-128, PubMed 33277687 , DOI 10.1007/s12104-020-09994-z , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 50355 released on 2020-06-24
    Title 1H, 13C, 15N backbone and ILMVA methyl group assignment of the N-terminal RecA domain of the DEAD-box RNA helicase DbpA from E.coli
  BMRB: 50356 released on 2020-06-24
    Title 1H, 13C, 15N backbone and ILMVAT methyl group assignment of the C-terminal RecA and RBD domains of the DEAD-box RNA helicase DbpA from E.coli

Related entities 1. full length DbpA, : 1 : 4 : 73 entities Detail

Interaction partners 1. full length DbpA, : 3 interactors Detail

More details for 3 interactors identified by 1 citations

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr50357_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr50357_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1811		91.0 (chain: 1, length: 457)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 457, Sequence coverage: 91.0%
- Total number of assignments
  - ¹H chemical shifts: 1150
  - ¹³C chemical shifts: 246
  - ¹⁵N chemical shifts: 415
- Completeness of assignments
  - Entity_assemble_ID: 1
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	925	412	44.5
¹³C chemical shifts	1338	55	4.1
¹⁵N chemical shifts	435	412	94.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	318	245	77.0
¹³C chemical shifts	318	246	77.4

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Found 1 Document (0.687 seconds)

BMRB: 50357

Sample

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. full length DbpA, : 1 : 4 : 73 entities Detail

Interaction partners 1. full length DbpA, : 3 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	2660	660	24.8
¹³C chemical shifts	2013	246	12.2
¹⁵N chemical shifts	506	415	82.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1735	248	14.3
¹³C chemical shifts	1099	246	22.4
¹⁵N chemical shifts	71	3	4.2